Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers

doi:10.3969/j.issn.1000-2006.2014.S1.001

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (增刊): 1-6.doi: 10.3969/j.issn.1000-2006.2014.S1.001

Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers

DUAN Yifan, WANG Xianrong^*, LIANG Lili, LI Xuexia, XIANG Qibai

International Cultivar Registration Center for Osmanthus, Nanjing Forestry University, Nanjing 210037, China

Online:1900-01-01 Published:1900-01-01

Abstract

Abstract: In this study, fluorescence-labeled simple sequence repeat(SSR)markers were used to fingerprinting the 64 Osmanthus fragrans cultivars under cultivation in China. Eleven pairs of SSR primers screened amplified 143 alleles,with an average of 13 alleles per locus. The average of H_o, H_e, I, N_a and N_e was0.619 7, 0.848 3, 2.323 7, 17.833 3 and 8.228 2, respectively. The Asiaticus Group had a distance relationship with the remaining groups. Two of OF002, OF019, OSM63 and OSM63 could distinguish all of the O. fragrans cultivars. Sixty-four O. fragrans cultivars with different SSR fingerprints of each other could serve as the cultivars-specific patterns and as an important basis for cultivars identification.

CLC Number:

Q948
S685.13

DUAN Yifan, WANG Xianrong, LIANG Lili, LI Xuexia, XIANG Qibai. Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2014, 38(增刊): 1-6.

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Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers

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