Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers

DUAN Yifan, WANG Xianrong, LIANG Lili, LI Xuexia, XIANG Qibai

Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2014, Vol. 38 ›› Issue (增刊) : 1-6.

PDF(1649822 KB)
PDF(1649822 KB)
Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2014, Vol. 38 ›› Issue (增刊) : 1-6. DOI: 10.3969/j.issn.1000-2006.2014.S1.001

Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers

  • DUAN Yifan, WANG Xianrong*, LIANG Lili, LI Xuexia, XIANG Qibai
Author information +
History +

Abstract

In this study, fluorescence-labeled simple sequence repeat(SSR)markers were used to fingerprinting the 64 Osmanthus fragrans cultivars under cultivation in China. Eleven pairs of SSR primers screened amplified 143 alleles,with an average of 13 alleles per locus. The average of Ho, He, I, Na and Ne was0.619 7, 0.848 3, 2.323 7, 17.833 3 and 8.228 2, respectively. The Asiaticus Group had a distance relationship with the remaining groups. Two of OF002, OF019, OSM63 and OSM63 could distinguish all of the O. fragrans cultivars. Sixty-four O. fragrans cultivars with different SSR fingerprints of each other could serve as the cultivars-specific patterns and as an important basis for cultivars identification.

Cite this article

Download Citations
DUAN Yifan, WANG Xianrong, LIANG Lili, LI Xuexia, XIANG Qibai. Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers[J]. Journal of Nanjing Forestry University (Natural Sciences Edition). 2014, 38(增刊): 1-6 https://doi.org/10.3969/j.issn.1000-2006.2014.S1.001

References

[1] Castiglione S, Wang G, Damiani G, et al. RAPD fingerprints for identification and for taxonomic studies of elite poplar(Populus spp.)clones[J]. Theoretical and Applied Genetics, 1993, 87(1-2): 54-59.
[2] Kopp R F, Smart L B, Maynard C A, et al. Predicting within-family variability in juvenile height growth of Salix based upon similarity among parental AFLP fingerprints[J]. Theoretical and Applied Genetics, 2002, 105(1): 106-112.
[3] Muhammad H R, Rajora O P. Microsatellite DNA fingerprinting, differentiation, and genetic relationships of clones, cultivars, and varieties of six popular species from three sections of the genus Populus[J]. Geonme, 2002, 45(6): 1083-1094.
[4] Rajora O P, Rahman M. Microsatellite DNA and RAPD fingerprinting, identification and genetic relationships of hybrid poplar(Populus canadensis)cultivars[J]. Theoretical and Applied Genetics, 2003, 106(3): 470-477.
[5] Fossati T, Zapelli I, Bisoffi S, et al. Genetic relationships and clonal identity in a collection of commercially relevant poplar cultivars assessed by AFLP and SSR[J]. Tree Genetics & Genomes, 2005, 1(1): 11-20.
[6] Gao J M, Zhang S G, Qi L W, et al. Application of ISSR markers to fingerprinting of elite cultivars(varieties/clones)from different section of the genus Populus[J]. Silvae Genetica, 2006, 55(1): 16.
[7] 李汝玉, 宋国安, 杨平平. 利用SSR标记进行品种权有争议品种的鉴定[J]. 种子, 2000(1): 66-67.Li R Y, Song G A, Yang P P. Identification of controversial cultivars using SSR markers[J]. Seed, 2000(1): 66-67.
[8] Yamamoto T, Kimura T, Sawamura Y, et al. SSRs isolated from apple can identify polymorphism and genetic diversity in pear[J]. Theoretical and Applied Genetics, 2001, 102(6-7): 865-870.
[9] Kimura T, Sawamura Y, Kotobuki K. Parentage analysis in pear cultivars characterized by SSR markers[J]. Journal of the Japanese Society for Horticultural Science, 2003, 72(3):182-189.
[10] Kimura T, Shi Y Z, Shoda M, et al. Identification of Asian pear varieties by SSR analysis[J]. Breeding Science, 2002, 52(2): 115-121.
[11] 梁海永, 刘彩霞, 刘兴菊, 等. 杨树品种的SSR分析及鉴定[J]. 河北农业大学学报, 2005, 28(4): 27-31.Liang H Y, Liu C X, Liu X J, et al. Simple sequence repeat(SSR)analysis and identify of different cultivars in Populus L.[J]. Journal of Agricultural University of Hebei, 2005, 28(4): 27-31.
[12] 韩宏伟, 杨敏生, 徐兴兴, 等. 利用SSR标记鉴定主要梨栽培品种[J]. 中国农学通报, 2006, 22(12): 383-386.Han H W, Yang M S, Xu X X, et al. Identification on the main cultivated varieties of Pyrus using SSR DNA marker[J]. Chinese Agricultural Science Bulletin, 2006, 22(12): 383-386.
[13] 张东, 舒群, 滕元文, 等. 中国红皮砂梨品种的SSR标记分析[J]. 园艺学报, 2007, 34(1): 47-52.Zhang D, Shu Q, Teng Y W, et al. Simple sequence repeat analysis on genetic assessment of Chinese red skinned sand pear cultivars[J]. Acta Horticulturae Sinica, 2007, 34(1): 47-52.
[14] 陈琛, 张兴桃, 程斐, 等. 秋甘蓝品种的SSR指纹图谱的构建[J]. 园艺学报, 2011, 38(1): 159-164.Chen C, Zhang X T, Cheng F, et al. Establishment of SSR fingerprinting on autumn cabbage hybrids and their parents[J]. Acta Horticulturae Sinica, 2011, 38(1): 159-164.
[15] 段艳凤, 刘杰, 卞春松, 等. 中国88个马铃薯审定品种SSR指纹图谱构建与遗传多样性分析[J]. 作物学报, 2009, 35(8): 1451-1457.Duan Y F, Liu J, Bian C S, et al. Construction of fingerprinting and analysis of genetic diversity with SSR markers for eighty-eight approved potato cultivars(Solanum tuberosum L.)in China[J]. Acta Agronomica Sinica, 2009, 35(8): 1451-1457.
[16] 麻丽颖, 孔德仓, 刘华波, 等. 36份枣品种SSR指纹图谱的构建[J]. 园艺学报, 2012, 39(4): 647-654.Ma L Y, Kong D C, Liu H B, et al. Construction of SSR Fingerprint on 36 Chinese jujube cultivars[J]. Acta Horticulturae Sinica, 2012, 39(4): 647-654.
[17] 莫文娟, 袁德义, 段经华, 等. 新高系梨9个品种SSR标记分析[J]. 浙江林学院学报, 2009, 26(5): 639-643.Mo W J, Yuan D Y, Duan J H, et al. Simple sequence repeat analysis on pear cultivars in Niitaka line[J]. Journal of Zhejiang Forestry College, 2009, 26(5): 639-643.
[18] 全志武, 汪静, 潘磊, 等. 10个藕莲品种SSR指纹图谱的构建与品种鉴别[J]. 中国蔬菜, 2008(3): 15-17.Quan Z W, Wang J, Pan L, et al. Construction of fingerprinting map and identification of cultivars for ten rhizome lotus cultivars by SSR markers[J]. China Vegetables, 2008(3): 15-17.
[19] 王通强, 马晓峰, 吴有祥. 油菜杂种及亲本指纹图谱构建和杂种纯度鉴定[J]. 食品与生物技术学报, 2009, 28(3): 377-384.Wang T Q, Ma X F, Wu Y X. Fingerprints construction of hybrid parents in brassica napus of the series of Guiza and its utilization in hybrid purity test[J]. Journal of Food Science and Biotechnology, 2009, 28(3): 377-384.
[20] 姚全胜,詹儒林,黄丽芳,等.11个芒果品种指纹图谱的构建与品种鉴别[J].热带作物学报,2009,30(11):1573-1576.Yao Q S, Zhan R L, Huang L F, et al. Construction of fingerprinting map and identification of cultivars for 11 mango cultivars by SSR markers[J]. Chinese Journal of Tropical Crops, 2009, 30(11): 1573-1576.
[21] 郝晨阳, 王兰芬, 贾继增, 等. SSR荧光标记和银染技术的比较分析[J]. 作物学报, 2005, 31(2): 144-149.Hao C Y, Wang L F, Jia J Z, et al. Comparison of fluorescence and silver-staining detection systems of microsatellite markers[J]. Acta Agronomica Sinica, 2005, 31(2): 144-149.
[22] 易红梅, 王凤格, 赵久然, 等. 玉米品种SSR标记毛细管电泳荧光检测法与变性PAGE银染检测法的比较研究[J]. 华北农学报, 2006, 21(5): 64-67.Yi H M, Wang F G, Zhao J R, et al. Comparison of two maize SSR detection methods: capillary electrophoresis with fluorescence detection method and denaturing PAGE silver-staining detection method[J]. Acta Agriculturae Boreali-Sinica, 2006, 21(5): 64-67.
[23] Smith L M, Sanders J Z, Kaiser R J. Fluorescence detection in automated DNA sequence analysis[J]. Nature, 1986, 321: 674-679.
[24] 张博, 张露, 诸葛强, 等. 一种高效的树木总DNA提取方法[J]. 南京林业大学学报: 自然科学版, 2004, 28(1): 13-16.Zhang B, Zhang L, Zhu G Q, et al. A rapid and simple method of total DNA extraction from tree[J].Journal of Nanjing Forestry University: Natural Sciences Edition, 2004, 28(1): 13-16.
[25] Zhang Z R, Fan D M, Guo S Q, et al. Development of 29 microsatellite markers for Osmanthus fragrans (Oleaceae), a traditional fragrant flowering tree in China[J].American Journal of Botany, 2011,98(12): 356-359.
[26] Lewontin R C. The apportionment of human diversity[J]. Evolutionary Biology, 1972, 16: 381-398.
[27] Nei M. Analysis of gene diversity in subdivided populations[J]. Proceedings of the National Academy of Sciences, 1973, 70(12): 3321-3323.
[28] Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proceedings of the National Academy of Sciences, 1979, 76(10): 5269-5273.
[29] Nei M. Molecular Evolutionary Genetics[M]. New York: Columbia University Press,1987.
[30] Yeh F C, Yang R C, Boyle T. POPGENE: Microsoft Windows Based Freeware for Population Genetic Analysis Release 1.31[M]. Edmonto:University of Alberta, 1999.
[31] Peakall R, Smouse P. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research[J]. Molecular Ecology Notes, 2006, 6(1): 288-295.
[32] 胡绍庆, 张厚勇, 吴光洪, 等. 桂花品种分类系统研究[J]. 浙江大学学报:农业与生命科学版, 2005, 31(4): 445-448.Hu S Q, Zhang H Y, Wu G H, et al. Studies on resource and taxon of Osmanthus fragrans cultivars[J]. Journal of Zhejiang University: Agriculture & Life Sciences, 2005, 31(4): 445-448.
[33] 向其柏, 刘玉莲. 中国桂花品种图志[M]. 杭州: 浙江科学技术出版社, 2008.Xiang Q B, Liu X L. An illustrated monograph of the sweet Osmanthus cultivars in China[M]. Hangzhou: Zhejiang Science and Technology Press, 2008.
[34] Yuan W J, Han Y J, Dong M F, et al. Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars[J]. Journal of Zhejiang University: Agriculture & Life Sciences, 2005, 31(4): 445-448.
[33] 向其柏, 刘玉莲. 中国桂花品种图志[M]. 杭州: 浙江科学技术出版社, 2008.Xiang Q B, Liu X L. An illustrated monograph of the sweet Osmanthus cultivars in China[M]. Hangzhou: Zhejiang Science and Technology Press, 2008.
[34] Yuan W J, Han Y J, Dong M F, et al. Assessment of genetic diversity and relationships amongltivars using AFLP markers[J]. Electronic Journal of Biotechnology, 2011, 14(1):2-3.
PDF(1649822 KB)

Accesses

Citation

Detail

Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

/