Abstract: Drought is one of the most stressful conditions limiting plant yield around the world. However,plants have developed multiple strategies to cope with environmental constraints. This study aimed to investigate the ability of Robinia pseudoacacia L.(2 years old)to regulate the process of water transportation in vivo under condition of drought and re-watering. Measurements of water transportation process parameters(water potential of leaves,leaf specific conductivity,relative water content of leaves and water consumption of seedlings)were made in R. pseudoacacia(2 years old)under condition of drought and re-watering.Results showed that:①R. pseudoacacia seedlings(2 years old)water transportation process stress index(Si)with the degree of drought stress increased and Si among different treatments was significantly different(P<0.05). ②Re-watering after drought stress 24 h,R. pseudoacacia seedlings(2 years old)water transport transportation process parameters had different degree of recovery,restoration degree(Rd)decreases with the increase of the degree of drought stress and Rd among different treatments was significantly different(P<0.05).③Re-watering after drought stress 24 h,compensation effect of R. pseudoacacia seedlings(2 years old)water transportation process was seen in the early growth stage of mild drought stress. Compensation index(Ci)decreased with the increase of drought stress. There were significant differences among different treatments(P<0.05).The conclusions were as follows:Drought stress could cause some damage to the water transportation process of plants,plants in order to maintain normal physiological activities,however,be able to start a series of stress response to prevent,reduce or repair the damage and the degree of recovery was closely related to growth period of seedlings and the degree of drought stress. This ability of seedlings to reduce or repair the injury was an essential index to evaluate the drought resistance and especially the selection of drought-resistant trees. Rewatering after 24 h,an obvious compensation effect of water transportation process was seen in R. pseudoacacia seedlings(2 years old),which was an important biological function to protect themselves,to a certain extent,could compensate for the injury of seedlings drought stress caused.