Endobacteria from different virulence Bursaphelenchus mucronatus

doi:10.3969/j.issn.1000-2006.2015.04.011

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (04): 63-68.doi: 10.3969/j.issn.1000-2006.2015.04.011

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Endobacteria from different virulence Bursaphelenchus mucronatus

YUAN Weimin^1,2, XIANG Yang^1,2, WU Xiaoqin^1,2*

1.Co-Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
2.Jiangsu Key Laboratory of Pest Prevention and Control,College of Forestry, Nanjing Forestry University,Nanjing 210037,China

Online:2015-07-31 Published:2015-07-31

Abstract

Abstract: Bursaphelenchus mucronatus is one of plant parasitic nematodes and similar to the causative agent of pine wilt disease B. xylophilus in morphology. To understand the interaction between B. mucronatus and endobacteria, ten strains of B. mucronatus from seven provinces were inoculated to 2-year old Pinus thunbergii to evaluate their virulence. Besides, endobacteria of B. mucronatus were also isolated and analyzed. Pathogenic measurement showed that six strains of B. mucronatus were pathogenic to P.thunbergii, but the other four strains had no pathogenicity. Seventeen strains of endobacteria were isolated from 9 of 10 B. mucronatus strains. Physiological and biochemical characteristics, biolog methods combined with molecular revealed that seventeen strains of endobacteria belonged to Stenotrophomonas maltophilia, Ewingella americana, Pseudomonas fluorescens, Agrobacterium tumefaciens,Luteibacter anthropi, Microbacterium oxydans, Agreia pratensis, Leifsonia naganoensis and Achromobacter xylosoxidans ssp. xylosoxidans. Further analysis showed that species and population of endobacteria strains from virulent B. mucronatus strains were more abundant than that from non-virulent strains. S. maltophilia were usually isolated from virulent B. mucronatus strains, while endobacteria M. oxydans were mainly isolated from avirulent strains.

CLC Number:

S763.16

YUAN Weimin, XIANG Yang, WU Xiaoqin. Endobacteria from different virulence Bursaphelenchus mucronatus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(04): 63-68.

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Endobacteria from different virulence Bursaphelenchus mucronatus

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