Genetic diversity and identification of pecan by ISSR markers

doi:10.3969/j.issn.1000-2006.2015.04.002

LI Hui, ZHANG Rui, PENG Fangren, LI Yongrong, ZHAI Min

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (04) : 7-12.

PDF(1813429 KB)

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (04) : 7-12. DOI: 10.3969/j.issn.1000-2006.2015.04.002

Genetic diversity and identification of pecan by ISSR markers

LI Hui¹, ZHANG Rui¹, PENG Fangren^1*, LI Yongrong², ZHAI Min²

Author information +

History +

Abstract

In this study, ISSR markers were used to evaluate the genetic diversity of 161 pecan cultivars and individuals, one walnut cultivar and one Carya cathayensis cultivars. A total of 56 bands were yielded by 14 ISSR premiers, and 55 were amplified bands(98% polymorphic). Nei’s gene diversity He and Shannon’s information index I were 0.366 8 and 0.542 8, respectively. The Nei’s genetic similarity coefficients ranged from 0.29 to 0.94, and the average was 0.649 6, which showed close genetic relationships. According to the results of cluster analysis, the 163 accessions could be divided into 9 groups, which showed that the genetic background was very complex.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LI Hui, ZHANG Rui, PENG Fangren, LI Yongrong, ZHAI Min. Genetic diversity and identification of pecan by ISSR markers[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(04): 7-12 https://doi.org/10.3969/j.issn.1000-2006.2015.04.002

References

[1] 彭方仁, 李永荣, 梁有旺, 等. 我国薄壳山核桃生产现状与产业化发展策略[J]. 林业科技开发, 2012, 4(1):1-4.Peng F R, Li Y R, Liang Y W, et al. Production status and industrialization development strategy of Carya illinoensis in China[J]. China Forestry Science and Technology, 2012, 4(1):1-4.
[2] 董凤祥, 王贵禧. 美国薄壳山核桃引种及栽培技术[M]. 北京: 金盾出版社, 2003.Dong F X, Wang G X. Pecan introduction and cultivation techniques[M]. Bejing: Jindun Publishing House, 2003.
[3] 吴国良, 张凌云, 潘秋红, 等. 美国山核桃及其品种研究性状[J]. 果树学报, 2003, 20(5): 404-409.Wu G L, Zhang L Y, Pan Q H, et al. Advances in study of pecan and its cultivar’s characteristics[J]. Journal of Fruit Science, 2003, 20(5): 404-409.
[4] 张日清, 李江, 吕芳德, 等. 我国引种美国山核桃历程及资源现状研究[J]. 经济林研究, 2003, 21(4): 107-109.Zhang R Q, Li J, Lv F D, et al. Historical survey of the introduced pecan tree in China: Distribution and resource estimate[J]. Economic Forest Researches, 2003, 21(4): 107-109.
[5] Conner P J, Wood B W. Identification of pecan cultivars and their genetic relatedness as determined by randomly amplied polymorphic DNA analysis[J]. J Amer Soc Hort Sci, 2001, 126: 474-480.
[6] 张日清, 何方, 吕芳德, 等. 美国山核桃群体遗传多样性的RAPD 分析[J]. 经济林研究, 2001, 19(2): 1-6. Zhang R Q, He F, Lv F D, et al. Population genetic analysis by randomly amplified polymorphic DNA markers in pecan [J]. Economic Forest Researches, 2001, 19(2): 1-6.
[7] 张日清, 吕德芳, 谭晓风, 等. 美国山核桃主要栽培样品的RAPD 鉴定[J]. 经济林研究, 2004, 22(4): 1-5.Zhang R Q, Lv F D, Tan X F, et al. Identification of main varieties of American hickory by RAPD [J]. Economic Forest Researches, 2004, 22(4): 1-5.
[8] Grauke L J, Iqbal M J, Reddy A S, et al. Developing mi-crosatellite DNA markers in pecan[J]. J Amer Soc Hort Sci, 2003, 128(3): 374-380.
[9] 佟兆国, 王富荣, 章镇, 等. 一种从果树成熟叶片提取DNA的方法[J]. 果树学报, 2008, 25(1): 122-125.Tong Z G, Wang F R, Zhang Z, et al. A method for DNA extraction from mature leaves of fruit tress[J]. Journal of Fruit Science, 2008, 25(1): 122-125.
[10] 于小玉, 喻方圆, 刘建兵, 等. ISSR在油茶品种鉴别和遗传多样性分析中的应用[J]. 南京林业大学学报:自然科学版, 2013, 37(1): 61-66.Yu X Y, Yu F Y, Liu J B,et al. Identification and genetic diversity analysis of Camellia oleifera varieties using ISSR marker[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2013, 37(1): 61-66.
[11] 彭方仁, 吴莺莺, 郝明灼, 等. 利用ISSR和SRAP标记分析油茶遗传多样性[J]. 南京林业大学学报: 自然科学版, 2012, 36(5): 19-25.Peng F R, Wu Y Y, Hao M Z,et al. Genetic diversity of Camellia oleifera using ISSR and SRAP markers[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2012, 36(5):19-25.
[12] 马红勃, 祁建民, 李延坤, 等. 烟草SRAP和ISSR分子遗传连锁图谱构建[J]. 作物学报, 2008, 34(11): 1958-1963.Ma H B, Qi J M, Li Y K, et al. Construction of a molecular genetic linkage map of tobacco based on SRAP and ISSR markers[J]. Acta Agronomica Sinica, 2008, 34(11): 1958-1963.
[13] 张晨东, 杨志新, 张聪, 等. 44份香料烟品种ISSR遗传多样性分析[J]. 热带作物学报, 2012, 33(12): 2144-2149.Zhang C D, Yang Z X, Zhang C, et al. ISSR analysis of genetic diversity for 44 oriental tobacco cultivars[J]. Chinese Journal of Tropical Crops, 2012, 33(12): 2144-2149.
[14] 施娟娟, 叶生月, 俞世群, 等. 37个新引进的薄壳山核桃品种遗传多样性SSR分析[J]. 安徽农业大学学报, 2013, 40(1):42-46.Shi J J, Ye S Y, Yu S Q,et al. SSR analysis of genetic diversity of the 37 new pecan cultivars[J]. Journal of Anhui Agricultural University, 2013, 40(1):42-46.
[15] 刘广勤, 王鹏良, 周蓓蓓, 等. 薄壳山核桃SRAP标记体系的优化和遗传多样性分析[J]. 江苏农业学报, 2010, 26(5): 1037-1042.Liu G Q, Wang P L, Zhou B B, et al. Optimization of SRAP marker reaction system and genetic diversity for Carya illinoensis Koch[J]. Jiangsu Journal of Agricultural Sciences, 2010, 26(5): 1037-1042.
[16] Jia X D, Wang T, Zhai M,et al.Genetic diversity and identification of Chinese-Grown pecan using ISSR and SSR markers [J].Molecules, 2011, 16(12): 10078-10092.
[17] 刘晓丽, 陈学森, 张美勇, 等. 普通核桃(Juglansregia)3个群体遗传结构的SSR分析[J]. 果树学报, 2008, 25(4):526-530.Liu X L, Chen X S, Zhang M Y,et al. Population genetic structure analysis of Juglans regia using SSR makers[J]. Journal of Fruit Science, 2008, 25(4): 526-530.
[18] 肖志娟, 翟梅枝, 许静, 等. 不同核桃品种的ISSR标记分析[J]. 北方园艺, 2013(4): 103-107.Xiao Z J, Zhai M Z, Xu J, et al. Analysis of different Walnut Varieties by ISSR Marker[J]. Northern Horticulture, 2013(4): 103-107.

PDF(1813429 KB)

Accesses

Citation

Detail

Sections

Recommended

The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

〈

〉

Please choose a citation manager

Content to export