JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 40 ›› Issue (01): 155-161.doi: 10.3969/j.issn.1000-2006.2016.01.025

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Application review of microscopic techniques on the investigation of lignocellulosic biomass pretreatment process

WANG Yan,JIN Yongcan*   

  1. Jiangsu Provincial Key Laboratory of Pulp and Paper Science and Technology, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, China
  • Online:2016-02-18 Published:2016-02-18

Abstract: The network structure which is formed by the components(cellulose, hemicellulose and lignin)of lignocellulosic biomass cell wall is believed to be the natural recalcitrance in the process of biomass conversion. A proper pretreatment is an efficient pathway to break this recalcitrance and enhance the conversion yield of enzymatic hydrolysis. Microscopic techniques including microscopy and microspectroscopy can be used to observe the microstructure of cell wall and analyze the change of component characteristics at multi-scales. The application of microscopic techniques, such as AFM, SEM, TEM and Raman microscopy on the process study of lignocellulosic biomass pretreatment, was briefly introduced in this review. The morphological change of cell wall surface and its effect on the enzymatic hydrolysis can be observed by microscopy technique. Microspectroscopy technique can be applied on in-situ analysis of component and ultrastructure changes of the cell wall during the pretreatment. The combination of multi-microscopic techniques covers the shortage of mono-technique, which can obtain the detail information on the biomass structure, as well as the content and distribution of cell wall components.

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