Effects of substrates on soil microbial carbon, nitrogen and phosphorus based on soil replacement method

doi:10.3969/j.issn.1000-2006.2017.02.011

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (02): 73-80.doi: 10.3969/j.issn.1000-2006.2017.02.011

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Effects of substrates on soil microbial carbon, nitrogen and phosphorus based on soil replacement method

ZHAO Juan, WANG Xingchang

Center for Ecological Research, Northeast Forestry University, Harbin 150040,China

Online:2017-04-18 Published:2017-04-18

Abstract

Abstract: 【Objective】Examine the effects of soil substrates on microbial biomass carbon(n_Cmic)nitrogen(n_Nmic), and phosphorus(n_Pmic)by comparing their values in the early stage of vegetation succession. 【Method】By using the soil displacement experiment performed included three treatments. Treatment A involved replacing the original soil of experimental plots(0-30 cm depth)with the A-horizon soil of an adjacent temperate broadleaved stand, whereas Treatment B involved replacing the soil with B-horizon soil, and treatment C involved replacing the soil with C-horizon soil. The three treatments simulated the secondary succession of clear-cut forests or bare soil without a seed bank or primary succession, respectively, and four replicates were used for each treatment. This study compared the difference of the n_Cmic, n_Nmic, n_Pmic, and their stoichiometry in different soil substrate. 【Result】① In 2004, the n_Cmic, n_Nmic and n_Pmicof treatment A were significantly greater than those of treatments B or C(P < 0.05), and the n_Cmic/n_Nmic ratio of treatment A was lower than those of treatments B or C, whereas the n_Cmic/n_Pmic and n_Nmic/n_Pmic ratios of treatment A were greater than those of treatments B or C. The n_Cmic/n_C_soil(soil carbon), n_Nmic/n_N_soil(soil nitrogen)and n_Pmic/n_P_soil(soil phosphorus)values of treatment A were significantly greater than those of treatments B or C(P < 0.05), except for the n_Nmic/n_N_soil value of treatment C. ② In 2014, the n_Cmic, n_Nmic and n_Pmic of treatment A were significantly greater than those of treatments B or C(P < 0.05), and the n_Cmic/n_Nmic ratio of treatments A and B were lower than that of treatment C, whereas the n_Cmic/n_Pmic and n_Nmic/n_Pmic ratios of treatment A were greater than those of treatments B or C. The n_Cmic/n_C_soil, n_Nmic/n_N_soil, and n_Pmic/n_P_soil values of treatment A were greater than those treatments B or C. ③ After the 10-year succession, the n_Cmic, n_Nmic and n_Pmic of the three treatments increased, except for the n_Pmic of treatment B. The n_Cmic/n_Nmic ratio of the three treatments declined, but the n_Cmic/n_Pmic and n_Nmic/ n_Pmic ratios increased. Among the three treatments, the n_Cmic/n_Nmic ratio of treatments B and C decreased significantly(P < 0.05), whereas the n_Nmic/n_Pmic ratio of treatments A and C increased significantly(P < 0.05). The n_Cmic/n_C_soil, n_Nmic/n_N_soil and n_Pmic/n_P_soil values of three treatments also increased with the succession. 【Condusion】 The physical and chemical properties of the different substrates were the main determinants of the spatial patterns of n_Cmic, n_Nmic, n_Pmic, and their stoichiometry.

CLC Number:

S718.5

ZHAO Juan, WANG Xingchang. Effects of substrates on soil microbial carbon, nitrogen and phosphorus based on soil replacement method[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2017, 41(02): 73-80.

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Effects of substrates on soil microbial carbon, nitrogen and phosphorus based on soil replacement method

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