Prediction of secretory protein in walnut bacterial black spot pathogen

ZHU Youpeng, LIU Hongli, HAN Changzhi

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (03) : 17-22.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (03) : 17-22. DOI: 10.3969/j.issn.1000-2006.201807003

Prediction of secretory protein in walnut bacterial black spot pathogen

  • ZHU Youpeng1, LIU Hongli1, HAN Changzhi1,2*
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Abstract

【Objective】 Fully understand the infection mechanism of black spot pathogen of walnut. 【Method】 The genome sequence of seven bacterial pathogens causing black spot of walnut, CFBP2528, CFBP7179, CFBP8253, DW3F3, J303, NCPPB1447 and Xaj417, whose complete genome sequence is known, were analyzed. Secretory proteins were predicted in the genome sequence based on the features of secretory protein using the online prediction programs such as SignalP v4.1, ProtCompB v9.0, TMHMM v2.0, big-PI Fungal predictor, TargetP v1.1, and LipoP v1.0. Additionally, the amino acid composition and distribution, signal peptide length, and signal peptide cleavage site of the secretory proteins were analyzed. 【Result】 The average number of secretory proteins predicted in the pathogens causing bacterial black spot of walnut was 74. The length of secretory proteins was mostly in the range of 101-400 amino acid residues, which accounted for about 63.65% of the total predicted secretory proteins. The abundant amino acid residue in the signal peptide was alanine, which accounted for 22.04%, followed by leucine, which accounted for 19.27% of the total amino acid residues. The length of signal peptide was mostly in the range of 19-29 amino acids residues, which accounted for 79.62% of the total predicted signal peptides. The signal peptide cleavage site belonged to the A-X-A type. 【Conclusion】The prediction of secreted proteins in the bacterial black spot pathogen of walnut is effe-ctively realized, which provides a theoretical foundation for further analysis of the secretory protein functions in the pathogens causing bacterial black spot of walnut during the process of infection.

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ZHU Youpeng, LIU Hongli, HAN Changzhi. Prediction of secretory protein in walnut bacterial black spot pathogen[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2019, 43(03): 17-22 https://doi.org/10.3969/j.issn.1000-2006.201807003

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