Mechanism of Burkholderia pyrrocinia JK-SH007 growth-promoting to plant via siderophore-mediation

doi:10.3969/j.issn.1000-2006.201811058

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (6): 165-172.doi: 10.3969/j.issn.1000-2006.201811058

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Mechanism of Burkholderia pyrrocinia JK-SH007 growth-promoting to plant via siderophore-mediation

MIN Lijing¹^,²(), GUO Lu³, YE Jianren¹^,^*()

1. College of Forestry, Nanjing Forestry University, Co-Innovation Center for the Sustainable Forestry in Southern China,Nanjing 210037, China
2. College of Life Science, Huzhou University, Huzhou 313000, China
3. Huzhou First People’s Hospital, Huzhou University, Huzhou 313000, China

Received:2018-11-28 Revised:2019-03-27 Online:2019-11-30 Published:2019-11-30
Contact: YE Jianren E-mail:178341894@qq.com;jrye@njfu.edu.cn

Abstract

Abstract:

【Objective】We aimed to explore the mechanism by which Burkholderia pyrrocinia JK-SH007, a gram-negative bacterium, promotes plant growth; we explored the effects of siderophores on pathogenic fungal activity and plant growth. 【Method】 A sample of JK-SH007-siderophores was isolated and purified using column chromatography, and the siderophore structure was identified using high-performance liquid chromatography and liquid chromatography-mass spectrometry. We cultivated three pathogens that cause poplar canker (Cytospora chrysosperma, Phomopsis macrospora and Fusicoccum aesculi) with siderophore samples to measure pathogen inhibition, and recorded the dry weight of mycelium at the end of the cultivation period. We compared the effects of a JK-SH007-siderophore solution, a JK-SH007 bacterial re-suspension, and a standard siderophore, Deferroamine B solution (DFOB) on plant growth (seed germination, cucumber seedling growth and tomato fruiting).【Result】Compared to DFOB, the JK-SH007-siderophore sample was more effective at inhibiting fungal growth. The pathogenic fungi were inhibited by (89±0.9)%, (76±0.6)% and (90±0.7)%, forC. chrysosperma, P. macrospora and F. aesculin, respectively. Compared to the JK-SH007 re-suspension, the JK-SH007-siderophore solution resulted in better seed germination and higher seedling vigor; however, there were no significant differences for cucumber seedling growth or tomato fruiting. 【Conclusion】We applied a pure siderophore sample, extracted and purified using chromatography, to plants, and compared this with a bacterial re-suspension sample and a standard commercial product. The pure siderophore sample strongly promoted plant growth and the application of a pure siderophore solution can achieve better anti-fungal effects than DFOB, a result which should inform future commercial development of JK-SH007 as a bio-fertilizer.

Key words: Burkholderia pyrrocinia, siderophore characterization, antifungi activity, plant growth promotion

CLC Number:

S763

MIN Lijing, GUO Lu, YE Jianren. Mechanism of Burkholderia pyrrocinia JK-SH007 growth-promoting to plant via siderophore-mediation[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(6): 165-172.

Figures/Tables 6

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References 35

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处理 treatment	发芽率/% germination index		胚根长/cm radicle length		幼苗活力指数/cm SVI
处理 treatment	黄瓜 cucumber	番茄 tomato	黄瓜 cucumber	番茄 tomato	黄瓜 cucumber	番茄 tomato
无菌水(对照)	90.0 c	86.7 c	4.1 c	6.8 c	3.6 d	5.9 c
JK-SH007-重悬液	96.7 b	90.7 b	4.3 c	6.9 c	4.2 c	6.3 b
JK-SH007-嗜铁素	100 a	100 a	6.2 a	7.8 a	6.2 a	7.8 a
DFOB	100 a	87.5 c	5.6 b	7.2 b	5.6 b	6.3 b

处理 treatments	株高/cm total plant height	根长/cm root length	总叶绿素含量/ (mg·L^-1) total chlorophyll content	湿质量/g wet weight
处理 treatments	株高/cm total plant height	根长/cm root length	总叶绿素含量/ (mg·L^-1) total chlorophyll content	茎 stem	根 root	茎 stem	根 root	挂果数 No. of fruits	果质量/g fruit weight	果径/cm fruit diameter	糖含量/ (^oBx) degrees Brix
DFOB	55.31 b	5.24 c	2.95 a	93.42 b	4.22 a	8.42 c	0.63 c	16 c	70.52 d	5.19 b	3.88 c
JK-SH007-嗜铁素	62.78 a	6.16 b	2.67 b	124.51 a	3.82 c	9.45 a	0.89 a	18 b	84.53 c	5.33 a	4.24 ab
JK-SH007-重悬液	53.82 b	6.24 a	2.74 b	86.72 c	3.94 b	8.84 b	0.78 b	22 a	88.78 a	5.40 a	4.25 a
无菌水(对照)	43.18 c	4.34 d	1.74 c	83.46 d	2.93 d	7.62 d	0.56 d	17 bc	85.37 b	5.35 a	4.12 b

Mechanism of Burkholderia pyrrocinia JK-SH007 growth-promoting to plant via siderophore-mediation

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[1]	CHEN Feifei,HUANG Jinsi,WANG Xiyun,LIU Wanhui,YE Jianren. Cloning and expression analysis of polygalacturonase gene(BpPG) from Burkholderia pyrrocinia JK-SH007 [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2018, 42(04): 127-133.
[2]	DOU Chengyang,WANG Yang,ZHANG Yuefeng,JIANG Mingming,YE Jianren. An application of three somatotrophic bacteria on pear in Shanghai [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2017, 41(04): 186-190.