Response of photosynthetic fluorescence of the endangered plant Manglietia ventii seedlings to shade treatment

doi:10.3969/j.issn.1000-2006.201810008

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (6): 46-52.doi: 10.3969/j.issn.1000-2006.201810008

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Response of photosynthetic fluorescence of the endangered plant Manglietia ventii seedlings to shade treatment

XU Qing¹(), BI Hongyan¹, CUI Guangshuai¹, GUO Xiaorong¹, ZHOU Rui¹, SU Wenhua¹, OUYANG Zhiqin², ZHANG Guangfei¹^,^*()

1. Institute of Ecology and Geobotany, Yunnan University, Kunming 650091, China
2. Yunnan Institute of Environmental Science, Kunming 650034, China

Received:2018-10-09 Revised:2019-05-06 Online:2019-11-30 Published:2019-11-30
Contact: ZHANG Guangfei E-mail:2571248649@qq.com;gfzhang@ynu.edu.cn

Abstract

Abstract:

【Objective】Light is an important environmental factor in the growth and development of plants. It effects plant growth and development by regulating the biosynthesis of phytochrome and other metabolic pathways. Manglietia ventii, is an endemic plant distributed in western China, and listed in the second-class national list of key conservative plants. The seedlings of M. ventii were cultivated under different light levels. The photosynthetic characteristics and chlorophyll fluorescence parameters of the seedlings were measured to investigate the adaptation and physiological responses and photo-protective mechanisms to the different light environment. Results may provide insights into the conservation and propagation of the endangered species. 【Method】Ten seedlings with similar size were randomly selected into 3 groups. Then each group was cultivated in the following relative illumination rate (RI) levels (100%, 30% and 8% created with different layers of black shading net) respectively for 6 months. During the growth period, the seedlings were watered and provided with nutrients properly. The photosynthetic characteristics of 5 healthy seedlings randomly chosen from RI 100%, 30% and 8%, respectively, were measured with the Li-6400 XT portable photosynthetic-fluorescence analyzer (Licor-6400, Lincoln, USA). The photosynthesis value of each seedling was recorded by averaging the data from 2 matured and healthy leaves. The photosynthetic-photoresponse curve was measured at 8:30-11:30 am Before the diurnal variation, the maximum photochemical efficiency (F_v/F_m) of the PSⅡ photosystem Ⅱ (PSⅡ) of the seedlings under the three light conditions were measured after dark adaptation for 30 minutes. The diurnal variation of photosynthetic parameters, including net photosynthetic rate (P_n), transpiration rate (T_r), stomatal conductance (G_s) were measured from 7:00 am to 19:00, for 3 days without clouds. At the same time, chlorophyll fluorescence parameters were measured, including photochemical quenching coefficient (q_P) and non-photochemical quenching coefficient (q_N), The effective photochemical quantum yield (F_v'/F_m') and electron transport rate (ETR) were measured with the same leaves for the measurement of photosynthesis. 【Result】Results indicated the response of P_n of seedlings under different levels of shade to photosynthetic active radiation (PAR) is similar; however, maximum net photosynthetic rate (P_{n, max}), light saturation point (P_LSP) and light compensation point (P_LCP) decreased with the increase in the levels of shade levels, which implicated that seedlings of M. ventii can tolerate shady environment. The diurnal variation of photosynthetic rate of all the seedlings under different light levels showed single peak curves, andP_n, T_r and G_s decreased with the increase in levels of shade. There is no midday depression of photosynthesis, which showed the seedlings of M. ventii can adapt to the light variation under the growth condition of sufficient water and nutrients. The diurnal variation of q_N, F_v'/F_m', and ETR decreased with the increase in shading levels and showed single peak curves too. However, the diurnal variation of q_P increased with the increase in shading levels, which indicated that shading promoted photosynthesis and enhanced the utilization of light energy and stimulated the photochemical activity of mesophyll cells. Leaves may mainly adapt to the low light habitat by increasing the open ratio of PSⅡ reaction center and the proportion of heat dissipation. Meanwhile,F_v/F_m was raised with the decrease of light levels. The value of F_v/F_m of leaves exposed to RI 100% is lower than 0.8 (that is normal for most plants in nonstress condition), which showed the occurrence of strong light inhibition. It implicated that long-term strong light treatment could damage the photosynthetic components of M. ventii seedlings and reduce its photochemical activity. 【Conclusion】F_v/F_m in full light (RI 100%) was significantly lower than that in the shading treatments (RI 30%, RI 8%) (P<0.05,F = 18.979, df = 2), which illustrated that the shading treatments effectively weakened light suppression of the M. ventii seedlings. Conclusively, shading treatment is more favorable to the growth of M. ventii seedlings and the best growth of M. ventii seedlings was expected under RI 30%.

Key words: Manglietia ventii, shade, photosynthesis, transpiration, chlorophyll fluorescence parameter

CLC Number:

S718
Q945

XU Qing, BI Hongyan, CUI Guangshuai, GUO Xiaorong, ZHOU Rui, SU Wenhua, OUYANG Zhiqin, ZHANG Guangfei. Response of photosynthetic fluorescence of the endangered plant Manglietia ventii seedlings to shade treatment[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(6): 46-52.

Figures/Tables 5

Fig.1

Fig.2

Table 1

Table 2

Fig.3

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RI/%	P_n/ (μmol· m^-2·s^-1)	G_s/ (μmol· m^-2·s^-1)	L_s/%
100	3.870±0.472 c	0.066±0.008 c	0.315±0.539 a
30	3.540±0.300 b	0.045±0.012 a	0.369±0.342 a
8	2.572±0.178 a	0.049±0.006 b	0.322±0.290 b

Response of photosynthetic fluorescence of the endangered plant Manglietia ventii seedlings to shade treatment

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RI/%	指标 index	P_n	T_r
100	T_r	0.903^**
100	G_s	0.789^*	0.981^**
30	T_r	0.963^**
30	G_s	0.803^*	0.962^**
8	T_r	0.864^*
8	G_s	0.752	0.968^**

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