Analysis of genetic diversity and variation of growth traits of three superior provenances of Pinus massoniana (masson pine) in Guangxi

doi:10.3969/j.issn.1000-2006.201806039

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (6): 67-72.doi: 10.3969/j.issn.1000-2006.201806039

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Analysis of genetic diversity and variation of growth traits of three superior provenances of Pinus massoniana (masson pine) in Guangxi

FENG Yuanheng¹(), LI Huogen², YANG Zhangqi¹^,^*(), HUANG Yongli³, CHEN Hu¹

1. Guangxi Institute of Forestry Science Timber Forest Research Institute,Masson Pine Engineering Technology Research Center of SFA(State Forestry Administration),Nanning 530002,China
2. Key Lab of Forest Genetics and Biotechnology, Nanjing Forestry University,Ministry of Education, Nanjing 210037,China
3. Nanning Forestry Division, Nanning 530107,China

Received:2018-06-28 Revised:2019-07-20 Online:2019-11-30 Published:2019-11-30
Contact: YANG Zhangqi E-mail:nanyuan05@163.com;yangzhangqi@163.com

Abstract

Abstract:

【Objective】This paper describes the study of a test plantation of 25-year-old Pinus massoniana (masson pine) trees, in which gene expression and DNA variation were examined on three levels: provenance, location and stand. The purpose was to provide a valuable reference from which genetic breeding strategies can be developed.【Method】The SSR molecular marker was used to perform genetic structure analysis, and the Arlequin 3.5 software was used to estimate the relative weight of each level of variation according to the linear model. The stand growth data were analyzed statistically by the SAS analysis software based on a linear model, and the relative weight of each level of variation was estimated by the four-factor nested model.【Result】Genetic diversity analysis showed that there was no obvious difference between provenances or within the same provenance. The results of molecular ANOVA analysis showed that the genetic variation between provenances accounted for only 2.56% of the total variation, and different locations within a provenance accounted for 1.66%. Alternatively, differences between individuals among those locations tested accounted for 95.78% of the total variation. Analysis of the growth phenotype data also showed no significant difference in diameter at breast height (DBH) and volume character between provenances, between locations within a provenance, or between superior and normal stands within a location. 【Conclusion】ANOVA analyses of molecular and growth phenotype data revealed that the major genetic variation of masson pine in Guangxi exists among individuals within a stand. Therefore, it is better to develop superior individual plant selection criteria in a superior provenance. This conclusion provides valuable information that can be used in the formulation of genetic breeding strategies for masson pine.

Key words: masson pine (Pinus massoniana Lamb.), genetic variation, SSR, analysis of molecular variance

CLC Number:

S718.46

FENG Yuanheng, LI Huogen, YANG Zhangqi, HUANG Yongli, CHEN Hu. Analysis of genetic diversity and variation of growth traits of three superior provenances of Pinus massoniana (masson pine) in Guangxi[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(6): 67-72.

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References 19

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子代号 No. of offspring stands	N_a	N_e	I	H_o	H_e
1	2.38	1.51	0.48	0.33	0.29
2	2.25	1.57	0.48	0.32	0.30
3	2.19	1.51	0.46	0.33	0.29
4	2.13	1.53	0.45	0.30	0.28
5	2.31	1.60	0.52	0.30	0.31
6	2.38	1.50	0.48	0.28	0.29
7	2.06	1.36	0.35	0.17	0.21
8	2.25	1.41	0.38	0.17	0.22
9	2.00	1.43	0.37	0.23	0.22
10	2.25	1.55	0.47	0.28	0.29
11	1.94	1.44	0.38	0.26	0.23
12	2.00	1.51	0.42	0.23	0.26
13	2.19	1.48	0.43	0.28	0.26
14	1.94	1.54	0.40	0.27	0.25
15	2.19	1.46	0.40	0.26	0.24
16	2.19	1.53	0.42	0.27	0.25
17	2.06	1.57	0.45	0.30	0.28
18	2.06	1.48	0.39	0.26	0.24

种源 provenance	N_a	N_e	I	H_o	H_e	F_st	N_m
桐棉 Tongmian	2.75	1.55	0.51	0.31	0.30	0.014	17.76
古蓬 Gupeng	2.69	1.48	0.44	0.22	0.25	0.025	9.90
云开大山 Yunkai Mountain	2.69	1.52	0.44	0.27	0.26	0.014	18.10

分析材料 analysis materials	方差来源 variance source	自由度 df	平方和 sum of squares	方差分量 variance component	总百分比/% percent of total
3个种源 three provenances	种源 provenance	2	53.75	0.057	2.56
	种源内地点 location	6	39.27	0.037	1.66
	地点内个体 individual	531	2 267.35	2.150	95.78
	合计 total	539	2 360.37	2.240	100.00
桐棉种源 Tongmian provenance	种源内地点 location	2	11.91	0.001	0.05
	地点内林分 stand	3	17.46	0.060	2.47
	林分内个体 individual	174	827.82	2.360	97.48
	合计total	179	857.19	2.420	100.00
古蓬种源 Gupeng provenance	种源内地点 location	2	17.51	0	0
	地点内林分 stand	3	31.24	0.140	6.83
	林分内个体 individual	174	667.95	1.910	93.17
	合计total	179	716.69	2.050	100.00
云开大山种源 Yunkai Mountain provenance	种源内地点 location	2	9.85	0.005	0.24
	地点内林分 stand	3	12.67	0.041	1.98
	林分内个体 individual	174	710.22	2.040	97.78
	合计total	179	732.74	2.090	100.00

种源 /林分 provenances/stands	H/m	D_DBH/cm	V/m³	种源 /林分 provenances/stands	H/m	D_DBH/cm	V/m³
桐棉Tongmian	19.15	22.03	0.3	普通林分superior stands	18.23	20.31	0.286
古蓬Gupeng	18.46	20.15	0.29	优良林分common stands	18.31	20.38	0.291
云开大山Yunkai Mountain	17.95	19.75	0.27

生长性状 growth trait	方差来源 variance source	自由度 df	平方和 sum of squares	均方 mean square	F	P_r > F	方差分量 variance component	总百分比/% percent of total
树高 height	种源 provenance	2	26.29	13.150	0.53	0.61	0	0
	种源内地点 location	6	148.40	24.730	0.56	0.75	0	0
	地点内林分stand	9	399.52	44.390	5.37	0.000 1^**	1.200	25.09
	林分内区组block	36	297.53	8.260	2.69	<0.000 1^**	0.520	10.81
	区组内个体individual	486	1 495.63	3.080			3.080	64.11
胸径 DBH	种源provenance	2	103.15	51.580	1.89	0.23	0.130 0	0.54
	种源内地点location	6	164.15	27.360	1.45	0.30	0.140 0	0.57
	地点内林分stand	9	169.82	18.870	0.80	0.62	0	0
	林分内区组block	36	850.98	23.640	0.96	0.54	0	0
	区组内个体individual	486	11 953.00	24.600			24.60	98.89
材积 volume	种源provenance	2	0.06	0.032	0.93	0.45	0	0
	种源内地点location	6	0.21	0.035	1.00	0.48	0	0
	地点内林分stand	9	0.31	0.035	1.91	0.08	0.001	2.84
	林分内区组block	36	0.66	0.018	0.96	0.53	0	0
	区组内个体individual	486	9.18	0.019			0.019	97.16

Analysis of genetic diversity and variation of growth traits of three superior provenances of Pinus massoniana (masson pine) in Guangxi

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