Effects of several factors on rooting of cutting propagation of ancient Platycladus orientalias and the changes of nutritive material

doi:10.3969/j.issn.1000-2006.201903057

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (1): 63-71.doi: 10.3969/j.issn.1000-2006.201903057

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Effects of several factors on rooting of cutting propagation of ancient Platycladus orientalias and the changes of nutritive material

WEI Qianchun¹^,²(), JIANG Zeping²^,³, LIU Jianfeng¹^,², SHI Shengqing¹^,², ZHAO Xiulian¹^,², CHANG Ermei¹^,²^,^*()

1. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
2. State Forestry Administration Key Laboratory of Tree Breeding and Cultivation,Beijing 100091, China
3. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091,China

Received:2019-03-21 Revised:2019-09-03 Online:2020-02-08 Published:2020-02-02
Contact: CHANG Ermei E-mail:675801545@qq.com;changem@caf.ac.cn

Abstract

Abstract:

【Objective】 Improve the rooting rate and establish the wig cutting propagation of ancient Platycladus orientalis. 【Method】 In this study, a total of 3 treatment groups using three different concentrations (200-600 mg/L, 1 000-3 000 mg/L and 4 000-10 000 mg/L) of ABT1, NAA or IBA+NAA (mass ratio 1:1) were used to treat 5-, 100-, 300-, 700-year-oldPlatycladus orientalis cuttings. The rooting process, rooting (rooting rate, rooting number, the longest root length) and the dynamic changes in nutrient content (soluble sugar, soluble starch) were observed during the rooting process of Platycladus orientalis cuttings. 【Result】 The observations revealed that 0-35 days was the formation stage, the 35-65 days was adventitious roots formation stage, and 65-95 days was adventitious roots elongation stage. The southern upper cuttings from the 50-year-old P. orientalis exhibited the best rooting percentage of 40.8%. The rooting rate of the 100-year-old P. orientalis was the highest in June with 20.1%. Low concentration growth regulator treatment of ABT1 (600 mg/L) for 4 h was beneficial to root formation of cuttings from 5-year-old P. orientalis, and a mixture solution of medium concentration IBA+NAA(moss ratio 1:1) treatment is good for rooting of cuttings from 100-, 300- and 700-year-old P. orientalis. Further studies revealed that the optimal mixture solution of IBA+NAA (moss ratio 1:1) of 1 000 mg/L for 1 min could improve the rooting rate of cuttings from the ancient P. orientalis. For all age groups, the content of soluble sugar and starch increased during callus formation stage; the 5-year-old P. orientalis showed the maximum increase. 【Conclusion】 Nutrient content of the branches are relatively higher in summer, which is beneficial for increasing the rooting rate of P. orientalis cuttings.

Key words: Platycladus orientalias, ancient trees, cutting time, cutting position, growth regulator treatment, nutritive material

CLC Number:

S792

WEI Qianchun, JIANG Zeping, LIU Jianfeng, SHI Shengqing, ZHAO Xiulian, CHANG Ermei. Effects of several factors on rooting of cutting propagation of ancient Platycladus orientalias and the changes of nutritive material[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(1): 63-71.

Figures/Tables 12

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References 30

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类别 type	处理/ (mg·L^-1) treatment concentration	浓度编号 No.	生长调节剂 growth regulator	调节剂编号 No.	处理时间 treatment time	时间编号 No.
低浓度 low soncen- tration	200	B₁	ABT1	A₁	1 h	C₁
	400	B₂	NAA	A₂	2 h	C₂
	600	B₃	IBA+ NAA (1∶1)	A₃	4 h	C₃
中浓度 middle soncen- tration	1 000	D₁	ABT1	A₁	30 s	E₁
	2 000	D₂	NAA	A₂	1 min	E₂
	3 000	D₃	IBA+ NAA (1∶1)	A₃	3 min	E₃
高浓度 high soncen- tration	4 000	F₁	ABT1	A₁	5 s	G₁
	8 000	F₂	NAA	A₂	30 s	G₂
	10 000	F₃	IBA+ NAA (1∶1)	A₃	60 s	G₃

扦插月份 cutting month	处理 treatments	生根率/% rooting rate	生根数/ 条 rooting number	最长根长/cm the longest root length
4	IBA+ NAA (质量比1∶1) 1 000 mg/L 1 min	14.7 b	3.5 bc	10.3 b
6		20.1 a	7.2 a	15.8 a
7		15.0 b	5.2 ab	7.0 c
8		8.7 c	2.5 c	1.8 d
9		5.1 c	1.2 c	1.5 d

采条部位 position	生根率/% rooting rate	生根数/条 rooting number	最长根长/m the longest root length
东上up of east	36.9 b	11.0 b	14.0 b
东下below of east	19.0 d	8.0 cd	13.3 bc
南上up of south	40.8 a	12.3 a	15.8 a
南下below of south	18.1 e	7.4 d	12.0 cde
西上up of west	24.6 c	8.9 c	12.4 bcd
西下below of west	16.1 e	6.7 d	12.9 bc
北上up of north	7.7 f	3.4 e	10.4 e
北下below of north	20.1 d	8.0 dc	10.9 de

处理 treatment	5 a		100 a		300 a		700 a
处理 treatment	生根率/% rooting rate	生根数/条 rooting number	生根率/% rooting rate	生根数/条 rooting number	生根率/% rooting rate	生根数/条 rooting number	生根率/% rooting rate	生根数/条 rooting number
A₁B₁C₂	73.9 b	20.2 c	19.0 a	5.0 b	16.1 b	2.2 c	0.0 d	0.0 e
A₁B₂C₃	38.4 f	12.7 e	0.0 c	0.00 f	5.5 e	0.8 d	3.9 c	0.7 d
A₁B₃C₁	90.2 a	23.2 b	0.0 c	0.0 f	20.1 a	2.8 b	7.7 bc	1.0 cd
A₂B₁C₁	66.5 c	17.3 d	13.2 b	3.3 bc	0.0 f	0.0 e	0.0 d	0.0 e
A₂B₂C₂	32.8 g	11.8 e	0.0 c	0.0 f	9.3 d	0.7 d	20.1 a	2.3 b
A₂B₃C₃	28.4 g	9.5 f	0.0 c	0.0 f	0.0 f	0.0 e	0.0 d	0.0 e
A₃B₁C₃	46.9 e	10.8 f	0.0 c	0.0 f	22.2 a	4.1 a	23.1 a	3.3 a
A₃B₂C₁	89.1 e	27.6 a	14.5 b	2.7 c	14.8 bc	2.2 c	16.1 b	1.7 c
A₃B₃C₂	67.8 c	15.2 d	14.8 b	4.3 b	0.0 e	0.0 e	0.0 d	0.0 e
CK	56.5 d	16.6 d	18.5 a	7.0 a	11.0 a	4.2 a	3.9 c	3.2 a

处理 treatment	5 a		100 a		300 a		700 a
处理 treatment	生根率/% rooting rate	生根数/条 rooting number	生根率/% rooting rate	生根数/条 rooting number	生根率/% rooting rate	生根数/条 rooting number	生根率/% rooting rate	生根数/条 rooting number
A₁D₁E₂	69.9 a	21.18 b	20.1 bc	5.83 b	17.2 bc	3.33 d	25.9 a	6.83 a
A₂D₁E₃	54.8 b	20.06 b	22.5 bc	5.67 b	18.5 bc	5.17 bc	14.8 b	2.00 cd
A₃D₁E₁	71.9 a	24.30 a	17.7 cd	5.50 b	29.3 a	7.17 a	18.5 c	3.83 b
A₁D₂E₁	26.5 e	16.22 bc	20.1 bc	7.17 ab	16.5 c	3.00 d	12.2 d	2.00cd
A₂D₂E₂	45.4 c	13.56 d	19.0 cd	5.17 b	0.0 d	0.00 e	0.0 e	0.00 e
A₃D₂E₃	47.7 c	11.77 de	26.5 a	8.83 a	20.1 b	3.83 cd	13.2 d	2.33 cd
A₁D₃E₃	48.5 c	15.94 bc	7.7 d	3.00 c	16.1 c	4.50 c	16.1 bc	2.50 c
A₂D₃E₁	20.1 f	9.36 e	21.4 bc	7.33 ab	17.7 bc	4.50 c	17.7 bc	3.17 b
A₃D₃E₂	34.4 d	17.24 bc	25.4 a	7.17 ab	19.5 b	6.00 b	20.1 b	2.67 c

Effects of several factors on rooting of cutting propagation of ancient Platycladus orientalias and the changes of nutritive material

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处理因素 treatment factors	生根率/% rooting rate		生根数/条 rooting number		最长根长/m the longest root length
处理因素 treatment factors	F	P	F	P	F	P
枝条部位cutting position	5.894	0.002^**	3.272	0.023^*	0.677	0.690
扦插时间cutting time	17.383	0.000^***	9.407	0.002^**	42.055	0.000^***
树龄tree age	38.686	0.000^***	46.164	0.000^***
生长调节剂处理growth regulator treatment	5.906	0.038^*	4.436	0.066	-	-

因素 factors	水平 levels	生根率/% rooting rate	生根数/条 rooting number
树龄/a tree age	5	51.4 a	13.85 a
	100	14.4 b	4.20 b
	300	13.5 b	2.65 b
	700	9.9 b	1.77 b
生长调节剂处理 growth regulator treatment	ABT1	18.2 ab	4.51 ab
	NAA	16.0 b	3.00 b
	NAA+IAA	21.7 a	5.20 a

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