Preliminary investigations of vector beetle and nematode species in Pinus massoniana forest in Huangshan City

doi:10.12302/j.issn.1000-2006.202106041

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (4): 29-35.doi: 10.12302/j.issn.1000-2006.202106041

Special Issue: 松材线虫病致病机理与病害防治

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Preliminary investigations of vector beetle and nematode species in Pinus massoniana forest in Huangshan City

WANG Lichao¹(), SU Shengrong²(), CHEN Fengmao¹^,^*(), DONG Xiaoyan¹, TIAN Chenglian³, WANG Yang¹

1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
2. College of Life and Environmental Sciences, Huangshan University, Huangshan 245041, China
3. Huangshan City Forestry Bureau of Auhui Province, Huangshan 245021, China

Received:2021-06-29 Revised:2021-10-10 Online:2022-07-30 Published:2022-08-01
Contact: CHEN Fengmao E-mail:921023280@qq.com;532884745@qq.com;cfengmao@126.com

Abstract

Abstract:

【Objective】The pine wilt disease is one of the serious forestry diseases in China, and causes rapid death in pine trees. To provide a theoretical support for the prevention and control of pine wood disease in Huangshan City, an investigation on the species of longicorn beetles and related nematodes was carried out in Pinus massoniana forest. 【Method】Longicorn beetles were collected indoors and outdoors from the Bocun forest farm. The beetles were fixed and identified, and their species and number were recorded. To confirm the presence of nematode vectors, Baermann funnel technique was used to extract the nematodes from beetles, which are harmful to the vitality of pine trees. After soaking the beetles for 12 h, a 15 mL centrifuge tube was used to place the soaking solution at the end of the funnel, which was then observed under a microscope to determine the presence or absence of nematodes. The ratio of beetles that carry nematodes was calculated by determining the ratio of nematode-containing beetles to the total number. To identify the species of nematodes carried by beetles, they were first cultured with fungi and preliminarily identified by observing their morphological characteristics. The molecular identification technique of Bursaphelenchus xylophilus was used to determine if the nematode was B. xylophilus. For the other parasitic nematodes, the 28S rDNA gene of nematodes was amplified, sequenced, blasted, and the phylogenetic tree was constructed for identification. Besides, the ratio and the number of nematodes carried by beetles at different times were counted. 【Result】 Total 14 longicorn beetles belonging to 12 genera and four subfamilies were collected. Two species of beetles Monochamus alternatus and Acanthocinus griseus were associated with nematodes, and their overall nematode carrying rates were 45.8% and 13.6%, respectively. M. alternatus and A. griseus both carry two types of dispersal nematodes with different morphological characteristics, one with a pointed tail and the other with a rounded tail. Results of the morphological and molecular analysis revealed the nematode with the pointed tail as B. xylophilus, while the other as Contortylenchus sp. During the period from June 19 to July 23, the weekly nematode isolation test results showed that the nematodes carried by female adults of M. alternatus were 76.5%, 66.0%, 52.9%, 34.7% and 10.0%, respectively; the proportion of nematodes carried by male adults of M. alternatus was 76.5%, 60.0%, 50.0%, 30.8% and 14.7%. The average number of nematodes carried by per individual female adult of M. alternatus was 1 018.0, 530.1, 202.5, 200.4 and 80.0, and the average number of nematodes carried by per individual male adult was 495.1, 68.3, 74.9, 8.2 and 3.8. 【Conclusion】 A total of 14 longicorn beetles were recorded in the masson pine forest in Bocun forest farm, Huangshan City. Among them, M. alternatus and A. griseus can carry B. xylophilus and Convertylenchus sp., the former being the main strain. The number and proportion of M. alternatus carrying nematodes gradually decreased with time.

Key words: Bursaphelenchus xylophilus, vector insect, Monochamus alternatus, Acanthocinus griseus, Pinus massoniana

CLC Number:

S763

WANG Lichao, SU Shengrong, CHEN Fengmao, DONG Xiaoyan, TIAN Chenglian, WANG Yang. Preliminary investigations of vector beetle and nematode species in Pinus massoniana forest in Huangshan City[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(4): 29-35.

Figures/Tables 5

Table 1

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诱捕天牛种类 species of longicorn beetles	数量/头 number of longicorn beetle trapped
松墨天牛 Monochamus alternatus	5 910
小灰长角天牛 Acanthocinus griseus	22
短角幽天牛 Spondylis buprestoides	3
赤梗天牛 Arhopalus unicalor	3
桃红颈天牛 Aromia bungii	2
星天牛 Anoplophora chinensis	1
八星粉天牛 Olenecamptus octopustulatus	1
弧纹虎天牛 Chlorophorus miwai	18
黄荆重突天牛 Tetraophthalmus episcopalis	1
桔狭胸天牛 Philus antennatus	2
斜顶天牛 Pseudoterinaea bicoloripes	1
斑角坡天牛 Pterolophia annulata	14
瘤胸簇天牛 Aristobia hispida	1
齿角坡天牛 Pterolophia serrata	1

Preliminary investigations of vector beetle and nematode species in Pinus massoniana forest in Huangshan City

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