Effects of polyacrylamide and urea peroxide compound oxygenator on the growth and physiological characteristics of hydroponic seedlings of Cymbidium ensifolium

doi:10.12302/j.issn.1000-2006.202204019

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2024, Vol. 48 ›› Issue (1): 115-123.doi: 10.12302/j.issn.1000-2006.202204019

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Effects of polyacrylamide and urea peroxide compound oxygenator on the growth and physiological characteristics of hydroponic seedlings of Cymbidium ensifolium

SONG Yumeng(), LI Suyan(), SUN Xiangyang

Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China

Received:2022-04-10 Revised:2022-05-31 Online:2024-01-30 Published:2024-01-24
Contact: LI Suyan E-mail:773169006@qq.com;lisuyan@bjfu.edu.cn

Abstract

Abstract:

【Objective】The purpose is to study the effects of compound oxygenating agent prepared with polyacrylamide (PAM) and urea peroxide on the growth and physiological characteristics of hydroponic Cymbidium ensifolium seedlings. 【Method】The seedlings of C. ensifolium were used as test materials; five treatments were set: CK (without PAM and urea peroxide, control), T1 [without PAM, 10% (mass fraction) urea peroxide], T2 [0.05%PAM, 10% urea peroxide], T3 (0.10%PAM, 10% urea peroxide) and T4 (0.20%PAM, 10% urea peroxide), to analyze the oxygen release characteristics of different oxygen release agents in water and their effects on growth, photosynthetic pigment, soluble protein content, root activity and antioxidant enzyme activity of hydroponic C. ensifolium seedlings in different periods. 【Result】The application of compound oxygenator prepared by PAM and urea peroxide can significantly increase dissolved oxygen in water in a certain period of time and enhance the slow-release effect. The root fresh weight and dry weight, leaf length, leaf width and root length were increased, root activity, photosynthetic pigment content, soluble protein content and POD enzyme activity were increased, which was beneficial to the growth of C. ensifolium seedlings treated with compound oxygenator. The survival days and SOD activity of the hydroponic C. ensifolium seedlings were significantly increased, under application of compound oxygenator treatments than the control when PAM content was higher (≥0.1%). The net photosynthetic rate of leaves and aerenchyma number of roots of hydroponic C. ensifolium seedlings were the highest when PAM supplemental level was 0.10%. 【Conclusion】That application of compound oxygenator prepared by PAM and urea peroxide can alleviate the damage to plants and improve the photosynthetic capacity and root activity of hydroponic C. ensifolium seedlings when PAM content is 0.10% by increasing dissolved oxygen concentration in water.

Key words: polyacrylamide (PAM), urea peroxide, compound oxygenator, hydroponics, Cymbidium ensifolium, hypoxia stress

CLC Number:

S682.31

SONG Yumeng, LI Suyan, SUN Xiangyang. Effects of polyacrylamide and urea peroxide compound oxygenator on the growth and physiological characteristics of hydroponic seedlings of Cymbidium ensifolium[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(1): 115-123.

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肥料 fertilizer	用量/ (mg·L^-1) dose	有效养分含量 content of available nutrients
硝酸钾(KNO₃)	607	含N 84.130 2 mg(13.86%),含K₂O 282.437 1 mg(46.53%)
磷酸二氢铵 (NH₄H₂PO₄)	115	含N 13.995 5 mg(12.17%),含P₂O₅ 71.001 mg(61.74%)
硫酸镁(MgSO₄)	493
四水硝酸钙〔Ca(NO₃)₂·4H₂O〕	945	含N 56.038 5 mg(5.93%)
总用量 total amount	2 160	总N 154.164 2 mg,总P₂O₅ 71.001 mg,总K₂O 282.437 1 mg; m(N)∶m(P₂O₅)∶m(K₂O) = 2.17∶1∶3.98

处理 treatment	根系干质量/g dry root mass	根系鲜质量/g root fresh mass	根系长度/cm root length	叶长/cm leaf length	叶宽/cm leaf width	成活时间/d survival days
CK	0.10±0.02 a	2.58±0.31 a	11.20±4.00 a	16.32±0.39 a	0.80±0.06 a	14±1.53 a
T1	0.10±0.02 a	2.71±0.49 ab	12.27±2.26 a	16.80±0.53 a	0.83±0.02 a	14±0.58 a
T2	0.12±0.08 a	2.92±0.16 ab	13.88±4.39 a	16.86±0.21 a	0.83±0.08 a	15±1.59 a
T3	0.13±0.06 a	3.36±0.12 ab	15.95±4.72 a	17.81±0.34 ab	0.92±0.11 ab	18±4.24 b
T4	0.14±0.02 a	3.56±0.67 b	15.85±3.66 a	18.01±0.40 b	0.97±0.03 b	18±2.00 b

处理 treatment	P_n/ (μmol· m^-2·s^-1)	C_i/ (μmol· mol^-1)	G_s/ (mol· m^-2·s^-1)	T_r/ (mmol· m^-2·s^-1)
CK	0.72 ± 0.04 d	209.73 ± 7.27 a	0.09 ± 0.01 a	1.42 ± 0.09 a
T1	0.93 ± 0.05 c	182.91 ± 5.82 b	0.07 ± 0.01 b	1.21 ± 0.13 a
T2	1.22 ± 0.09 b	158.65 ± 12.38 c	0.04 ± 0.01 c	0.78 ± 0.05 b
T3	1.33 ± 0.05 a	141.51 ± 7.07 c	0.02 ± 0.00 d	0.36 ± 0.26 c
T4	1.19 ± 0.02 b	160.54 ± 20.36 c	0.04 ± 0.01 c	0.69 ± 0.22 b

Effects of polyacrylamide and urea peroxide compound oxygenator on the growth and physiological characteristics of hydroponic seedlings of Cymbidium ensifolium

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