Abstract: The phosphatidylglycerol from the needles of different crosses of Chinese fir were isolated using our improved method of DEAE CL6B column chromatography and silica thin layer chromatography after being extracted with chloroform and methanol. A quantitative analysis was conducted by gas chromatography on a DBFFAP column. The results showed that there were main five compositions of fatty acid in phosphatidylglycerol in the needles of Chinese fir. IUFA could reflect the ralative ability to chilling of Chinese fir, and it could be considered as one of indexes to evaluate the ability of chilling_resistance.