JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2002, Vol. 26 ›› Issue (02): 73-76.doi: 10.3969/j.jssn.1000-2006.2002.02.020

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Tissue Culture and Plant Regeneration of Cerasus campanulata

WANG Guang ping,HUANG Min ren   

  1. The Key Laboratory of Forestry Genetics & Gene Engineering Nanjing Forestry University,Nanjing 210037,China
  • Online:2002-04-18 Published:2002-04-18

Abstract: Stabilized shoot cultures were achieved when the explant sources were the juvenile branches of Cerasus campanulata in spring.The inducing rate of fasciculated shoots was 87.5% when the inducing medium of 1/4MS with BA 1.0 mg/L and IBA 0.01 mg/L were used.The adventitious shoots were propagated most rapidly when subcultured on the medium of MS with BA 1.0 mg/L,IBA 0.1 mg/L and GA 3 0.3 mg/L,or MS with KT1.0 mg/L,NAA 0.1 mg/L and GA 3 0.3 mg/L.The elongation of adventitious shoots was greatly affected by gibberellin.The adventitious shoots were cut and rooted in the rooting medium of 1/2MS with NAA 1.0 mg/L IBA 1.0 mg/L and BA 0.75 mg/L.The tooting rate was more than 90%.The surviving rate was 95% when the intact shoots were moved to the greenhouse.

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