JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2003, Vol. 27 ›› Issue (03): 55-58.doi: 10.3969/j.jssn.1000-2006.2003.03.013

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RAPD Studies on the Amplified Product of Single Microdissected Cunninghamia lanceolata Chromosome

LI Xiangyang1,ZHOU Jian1*,YUAN Hansheng2,ZHANG Zailiang2   

  1. 1.Nanjing Forestry University,Nanjing 210037,China;2.Forestry Bureau of Yongzhou Hunan Province,Yongzhou 425000,China
  • Online:2003-06-18 Published:2003-06-18

Abstract: Four single chromosome of Cunninghamia lanceolata was dissected under the micromanipulator.After degenerate oligonucleotideprimedPCR amplification(DOPPCR),DNA product were tested with RAPD.The results indicated: the polymorphism among different single microdissected Cunninghamia lanceolata chromosome was evident.This research provides a new method to identify microdissected Cunninghamia lanceolata chromosome and locate gene to chromosome of Cunninghamia lanceolata chromsome.

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