JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2004, Vol. 28 ›› Issue (05): 37-40.doi: 10.3969/j.jssn.1000-2006.2004.05.009

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Efficiency of the Assistant Method on Agrobacterium-mediated Transformation of Japanese Lawngrass

HU Fan-rong~(1,2),XIA Ying-wu~2   

  1. 1.Jinhua College of Profession & Technology,Jinhua 321007,China;2.Zhejiang University,Hangzhou 310029,China
  • Online:2004-10-18 Published:2004-10-18

Abstract: The assistant method of agrobacterium-mediated transformable efficiency which includes irradiation,callus,co-cultivation and negative pressure,is explored with the callus of Japanese lawngrass(Zoysia japonica Steud.).The results are as follow:2?J/kg of irradiation-dosage benefits the exaltation of transformable efficiency;1~2 months of callus proper is fit to the transformation;the transformable efficiency of the whole light shine to co-cultivation is higher than that of 16?h and 8?h photoperiod and the whole dark;the proper negative pressure,when agrobacterium and callus cultivated,could increase transformable frequency and survival rate of callus.

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