Abstract: Aluminum(A1) toxicity is a limiting factor for agriculture in acidic soils where it results in an inhibition of root growth, drought intolerance, nutrient deficiencies and other physiological and biochemical changes in plants. Soybean is highly nutritious being particularly rich in protein content and useful for soil improvement. In present study,the seedlings of 7 soybean cultivars were grown in solution with or without 2 mmol/L A1 stress in a randomized complete block design experiment for 2 weeks, to evaluate the differential responses of soybean genotypes to AI stress on seedling growth. Results showed that epicotyl length,seedling height, root length, fresh and dry weights, and chlorophyll content were decreased for the cultivars tested under A1 stress. There was no uniform response of the cultivars to A1 stress in seedling growth. However,seven cultivars seem to be categorized as 3 groups,based on the relative root growth data as follows. Nannong 73-935 and Dahuangdou were the most tolerant (15% reduction) and Nannong 87C-38 and Williams were the most sensitive(37%～39% reduction); 1138-2, Hongyin No. 1 and Kefeng No. 1 (23%～27% reduction) showed intermediate response under A1 stress.