Sixty-five Chrysanthemum cultivars were analyzed. The genetic diversity. Six pairs of primer combinations generated 244 bands in total, in which 178 bands were polymorphic. The average percentage of polymorphic bands was 72.95%. Cluster analyses by UPGMA showed that there were close genetic relationships among cultivars of the same petal type. Their origin was correlated with the analysis cluster results at a certain level. But the flower color had no clear pertinence with the cluster results.