Preparation of monodisperse fluorescent microspheres and their optical properties

doi:10.3969/j.jssn.1000-2006.2010.03.004

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2010, Vol. 34 ›› Issue (03): 15-19.doi: 10.3969/j.jssn.1000-2006.2010.03.004

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Preparation of monodisperse fluorescent microspheres and their optical properties

WU Weibing¹, WANG Mingliang², JING Yi¹, DAI Hongqi¹

1.Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, China; 2.School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China

Online:2010-06-29 Published:2010-06-29

Abstract

Abstract: Fluorescent monodisperse polystyrene microspheres were prepared by dispersion polymerization by modified two stage dispersion polymerization in which polyvinylpyrrolidone with high molecular weight (Mw=300 000 g/mol) was used as the stabilizer and the dye was added in a relative long period after nucleation stage. Two stage dispersion polymerization combined with embedment method resulted in more uniform microspheres and higher dye concentration compared to adsorption and conventional dispersion polymerization method. The surface morphology, average diameter, size distribution and photoluminescence properties of microspheres were investigated. The found intense fluorescence and fluorescent resistance to the solvents indicate that the microsphere structure protects dye molecules from fluorescence quenching and ambient medium. This type of monodisperse fluorescent microspheres has potential application in bioassays.

CLC Number:

O632
TB34

WU Weibing, WANG Mingliang, JING Yi, DAI Hongqi. Preparation of monodisperse fluorescent microspheres and their optical properties[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2010, 34(03): 15-19.

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Preparation of monodisperse fluorescent microspheres and their optical properties

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