Apriona germari (Hope) was chosen as a model to investigate karyotype in this paper. Ganglions of larvae, ganglions, salivary glands, testis and ovaries of adults, tissues of embryo were used as experimental materials. The results showed that the optimal materials were 2, 3 days old embryos. The nucleic acid content of embryo was determined. The content was increasing with day age, and peak value appeared at 3, 6, 9 days old embryo. The ratio of RNA/DNA in 3 days old embryo was higher than that in 6 or 9days old embryo. When the nucleic acid peak value appeared firstly, the embryo was the most suitable material for the chromosome research. So the ratio of RNA/DNA in embryo could be used as an index of material choice for making chromosome specimen.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1]Smith S G. Chromosome numbers of Coleoptera[J]. Heredity, 1953(2): 31-48. [2]Vidal O R. Chromosome numbers of Coleoptera from Argentina[J]. Genetica, 1984, 65: 235-239. [3]Rozek M, Lachowska D, Petitpierre E, et al. Cbands on chromosomes of 32 beetle species(Coleoptera: Elateridae, Cantharidae, Oedemeridae, Cerambycidae, Anthicidae, Chrysomelidae, Attelabidae and Curculionidae)[J]. Hereditas, 2004, 140: 161-170. [4]Yadav J S, Pujai R K. Evolution of karyotypes and phylogenetic relationships in Scarabaeidae(Coleoptera)[J]. Zool Anz Jena, 1979, 202(1/2): 105-118. [5]杨致远,黄培,吴国雄. 米象、玉米象及其杂交后代染色体的观察研究[J]. 昆虫学报,1989,32(4):406-409. [6]邵红光,黄人鑫. 新疆6种芫菁染色体核型的研究[J]. 新疆大学学报:自然科学版,1994(3):81-86. [7]陈斌,杨本立,李正跃. 码绢金龟精巢染色体的制作观察技术[J]. 云南农业科技,2005(3):15. [8]董原,商晗武,崔旭红,等. 蓼蓝齿胫叶甲生物学特性和染色体核型研究[J]. 浙江农业科学,2008(4):424-428. [9]嵇保中,钱范俊,严敖金,等. 桑天牛成虫神经系统解剖[J]. 南京林业大学学报,1998,22(2):89-93. [10]李国珍. 染色体及其研究方法[M]. 北京:科学出版社,1995. [11]张韵梅. 测定昆虫卵巢内核酸、蛋白质等含量的方法研究[J]. 山东农业大学学报,1987,18(4):49-51. [12]伊淑霞,郭郛. 柞蚕卵巢中核酸和蛋白质的分布与含量的变化[J]. 昆虫学报,1984,27(3):254-263. [13]刘存歧,沈会涛,吴玲玲. 日本沼虾体内RNA/DNA比值与其生长关系的研究[J]. 河北大学学报:自然科学版,2006,26(5):524-528. [14]Lanier G N, Raske A G. Multiple sex chromosomes and configuration polymorphism in the Monochamus Scutellatus-oregonensis complex(Coleoptera: Cerambycidae)[J]. Can J Genet Cytol, 1970, 12: 947-951. [15]Teppner H. Chromosomenzahlen einiger mitteleuropischer Cerambycidae(Coleoptera)Ⅱ[J]. Chromosoma(Berl.), 1968, 25: 141-151.
PDF(637144 KB)
