Abstract: Thirteen ESTSSR molecular markers related to GAs metabolism for Pinus L. were developed from EST sequences in PGI（Pinus gene index）database. 1 014 alleles were amplified with two parents and 39 F1 progeny of Pinus taeda×P. caribaea var. hondurensis with four primerpairs, which were selected from the thirteen ESTSSRs. 5019 % of the 1 014 alleles were originated from the male parent（P. taeda） and 5217 % were shared with the female parent（P. caribaea var. hondurensis）, which showed that the progeny of this hybridization inherent genetic materials from both parents. Six locus selected from all 26 locus amplified by four primer pairs were related significantly to sixmonth old seedling height of the 39 F1 hybrids, and five locus related significantly to ninemonth old seedling height. Therefore, this markers can serve as good molecular markers for early selection.