ptimization of cellulase components by mixed culture of Trichoderma reesei and Aspergillus niger

doi:10.3969/j.jssn.1000-2006.2011.02.017

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2011, Vol. 35 ›› Issue (02): 78-82.doi: 10.3969/j.jssn.1000-2006.2011.02.017

Previous Articles Next Articles

ptimization of cellulase components by mixed culture of Trichoderma reesei and Aspergillus niger

SONG Xiangyang，FANG Hao，OUYANG Jia，LI Xin，YONG Qiang

Key Laboratory of Forest Genetic and Biotechnology Ministry of Education, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037 China

Online:2011-04-13 Published:2011-04-13

Abstract

Abstract: Mixed culture of Trichoderma reesei and Aspergillus niger was investigated to optimize the cellulase components in liquidstate fermentation. A mode of delayed inoculation was applied for cocultivation of T. reesei and A. niger. After cultivation of T. reesei for 48 h, A. niger was inoculated at the 1∶1 inoculum ratio. The βglucosidase activity and filter paper activity of the mixed fungi culture were 0.606 μmol/(min·mL) and 1.163 μmol/(min·mL), respectively, corresponding to a ratio of 0.521, which was higher than that of T. reesei under single cultivation. The yield of enzymatic hydrolysis was 65.61 % by the cocultivated cellulase, which was higher than those of singlecultivated cellulase and commercial cellulase. These results demonstrated that cocultivation of T. reesei and A. niger was suitable for optimization of cellulase components.

CLC Number:

TQ925

SONG Xiangyang，FANG Hao，OUYANG Jia，LI Xin，YONG Qiang. ptimization of cellulase components by mixed culture of Trichoderma reesei and Aspergillus niger[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2011, 35(02): 78-82.

References

[1]张继泉,王瑞明,孙玉英,等. 里氏木霉生产纤维素酶的研究进展[J].饲料工业， 2003,24(1):9-13.
[2]Sun Y，Cheng J. Hydrolysis of lignocellulosic materials for ethanol production: a review[J]. Bioresource Technology， 2002,83:1-11.
[3]Zhang Y H P，Lynd L R. Toward an aggregated understanding of enzymatic hydrolysis of cellulose: noncomplexed cellulase systems[J].Biotechnology and Bioengineering， 2004,88(7):797-824.
[4]Zhang Y H P，Himmel M E，Mielenz J R. Outlook for cellulase improvement: Screening and selection strategies[J]. Biotechnology Advances， 2006,24:452-481.
[5]Ortega N,Busto M D,PerezMateos M. Kinetics of cellulose saccharification by Trichoderma reesei cellulose[J]. International Biodeterioration & Biodegradation,2001,47(1):7-14.
[6]Stockton B C. Cellobiase production by a selected Aspergillus niger strain in solid state fermentation[J]. Biotechnology Letters,1991,13:57-62.
[7]Rashid M H,Rajoka M I,Siddiqui K S,et al. Kinetic properties of chemically modified βglucosidase from Aspergillus niger 280[J]. Pakistan Journal of Zoology,1997,29:354-363.
[8]Juhász T,Kozma K,Szengyel Z,et al. Production of βglucosidase in mixed culture of Aspergillus niger BKMF 1305 and Trichoderma reesei rut c-30[J]. Food Technology and Biotechnology,2003,41:49-53.
[9]Mandels M,Medeiros J E,Andreotti R E,et al. Enzymatic hydrolysis of cellulose: Evaluation of cellulase culture filtrates under use condition[J]. Biotechnology Bioengineering,1981,23:2009-2026.
[10]宋向阳,李鑫,毛连山,等.分批添料对戊糖、己糖同步发酵制备乙醇的影响[J]. 南京林业大学学报:自然科学版,2008,32(6):9-12.
[11]Hanif A,VasmeenA,Rajoka M I. Induction,production,repression,and derepression of exoglucanase synthesis in Aspergillus niger[J]. Bioresource Technology,2004,94:311-319.
[12]Wen Z,Liao W,Chen S. Production of cellulase/βglucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure[J]. Process Biochemistry，2005，40：3087-3094.
[13]Howell J A,Stuck J D.Kinetics of solka floc cellulose hydrolysis by Trichoderma viride cellulase[J].Biotechnology and Bioengineering,1975,17：873-893.
[14]Chen M，Zhao J，Xia L. Enzymatic hydrolysis of maize straw polysaccharides for the production of reducing sugars[J]. Carbohydrate Polymers, 2008,71:411-415.
[15]Fang H，Zhao C，Song X Y. Optimization of enzymatic hydrolysis of steamexploded corn stover by two approaches: Response surface methodology or using cellulase from mixed cultures of Trichoderma reesei RUTC30 and Aspergillus niger NL02[J]. Bioresource Technology, 2010,101:4111-4119.

Metrics

Comments

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

ptimization of cellulase components by mixed culture of Trichoderma reesei and Aspergillus niger

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Recommended Articles

Metrics

Comments

Author

Reader

Reviewer

[1]	SU Erzheng, LI Mingliang, WEI Dongzhi. Study on in situ immobilization and characterization of papain [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2014, 38(06): 99-104.
[2]	PEI Jianjun， L Xiangxiang， XIE Jingcong， ZHAO Linguo*. Inducement condition and immobilization of thermostable αglucosidase produced by Escherichia coli [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2012, 36(06): 90-94.