Abstract
By twodimension gel electrophoresis, differential proteomic analysis was conducted on Populus beijingensis(sensitive to Botryosphaeria dothidea) and P. tomentosa(resist to B. dothidea) before and after inoculated with B. dothidea（2DE）. Comparison of the 2DE maps of proteins from the two inoculated and control species. The results showed that 35 3fold different proteins were detected between the two species.Among which there were 4 upregulated proteins, 15 downregulated proteins before inoculation, and 7 upregulated proteins, 9 downregulated proteins after inoculation. For P. tomentosa, there were 8 upregulated proteins and 6 downregulated proteins detected after inoculation, and for P. beijingensis, 8 upregulated proteins and 10 downregulated proteins were identified after inoculation The analysis was taken on the produce conditions and contents of these proteins.It could be deduced that the upregulated proteins expressed in P. tomentosa before and after inoculation may be associated with consistutive resistance mechanisms and induced resistance mechanisms respectively. Four of them were detected by mass spectrometry, which were ribulose bisphosphate carboxylase small clain, predicted protein, superoxide dismutase, isopentenyldiphosphate deltaisomerase respectively. These proteins may be associated with the resistance of poplar to canken.
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LI Yongxia, L Quan, LIANG Jun, YAN Donghui, JIA Xiuzhen, ZHANG Xingyao.
Differential analysis of twodimensional gel electrophoresis profiles of protein in poplars after inoculation with Botryosphaeria dothidea[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2011, 35(04): 1-6 https://doi.org/10.3969/j.jssn.1000-2006.2011.04.001
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References
[1]张星耀，骆有庆．中国森林重大生物灾害[M]．北京：中国林业出版社，2003.
[2]梁军，姜俊清，刘会香，等． 我国杨树与溃疡病菌互作的病理学研究[J].林业科学研究，2005，18(2)：214-221.
[3]胡景江，朱玮． 溃疡病菌对杨树几丁酶、BN乙酰氨基葡萄糖苷酶的诱导作用[J].植物病理学报，1997，27(2)：181-185.
[4]胡景江，刘志龙，文建雷．溃疡病菌低聚糖激发子诱导杨树细胞抗病机制的初步研究[J].西北农林科技大学学报：自然科学版，2003，31(4)：145-148.
[5]梁军，王媛，张星耀． 杨树与溃疡病菌互作中过氧化物酶的细胞化学定位[J]．北京林业大学学报，2008，30(6)：107-111.
[6]沈瑞祥，项蔚华，林仲桂．5406细胞分裂素混合剂对杨树溃疡病抑制作用的研究[J]．植物病理学报，1988，18 (4)：251-254.
[7]杜建玲，项蔚华，沈瑞祥．诱导杨树抗溃疡病机理的研究[J]．林业科学研究， 1995，8(1)： 78-81.
[8]袁坤. 杨盘二孢菌侵染后杨树叶片差异表达蛋白质的鉴定[D].南京：南京林业大学，2007.
[9]杨俊秀，李武汉，符毓秦，等．抗溃疡病杨树种类的调查研究[J].西北林学院学报，1990，5(4): 1-10.
[10]阳传和，田砚亭．应用放射性同位素示踪法研究杨树溃疡病菌在杨树苗茎中的扩展与分布[J].北京林业大学学报,1986,8（2）:99-105.
[11]Damerval C,Vienne D D, Zivy M, et al. Technical improvements in twodimensional electrophoresis increase the level of genetic variation detected in wheatseedling proteins[J]. Electrophoresis, 1986,7(1): 52-54.
[12]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding[J].Anal Biochem, 1976, 72: 248-254.
[13]孟慧，段翠芳，曾日中．植物蛋白质组学研究概况[J]．热带农业科学，2006，26(2):60-64，82.
[14]刘卫群，李浩．差异蛋白质组学在植物研究中的应用[J]．安徽农业科学，2006，34(17):4201-4203.
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