Application of quadratic regressive factorial experiment to in vitro rooting culture of Catalpa bungei

doi:10.3969/j.jssn.1000-2006.2011.04.010

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2011, Vol. 35 ›› Issue (04): 47-50.doi: 10.3969/j.jssn.1000-2006.2011.04.010

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Application of quadratic regressive factorial experiment to in vitro rooting culture of Catalpa bungei

YU Yongming¹，WANG Junhui^2*，ZHANG Songzhi¹，MA Jianwei¹，LI Pingying¹

1.Xiaolongshan Research Institute of Forestry, Tianshui 741022, China；2.Chinese Academy of Forestry，Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration，Beijing 100091, China

Online:2011-08-13 Published:2011-08-13

Abstract

Abstract: Quadratic regressive factorial experiment and SPSS software was used to analyse and optimize concentrations of IBA and NAA added in rooting medium for the rooting rate，root length，root number，sprouting number，bud length，leaf number and shoot fresh weight of Catalpa bungei, we obtained a serial of regression equations. Using such equations to search for the best dosage of IBA and NAA to induce root in vitro culture of C. bungei. The results showed that, a certain concentration of NAA could promote the root differentiation and growth, and increase leaves, but there is no significant role to the growth of buds and sprouting number.The best dosage range of IBA is in 0.200—0.344 mg/L, and dosage range of NAA is in 0.023—0.073 mg/L, the rooting rate was achieved at 95 %, the probability of the event was 95 %.

CLC Number:

S718

YU Yongming，WANG Junhui，ZHANG Songzhi，MA Jianwei，LI Pingying. Application of quadratic regressive factorial experiment to in vitro rooting culture of Catalpa bungei[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2011, 35(04): 47-50.

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Application of quadratic regressive factorial experiment to in vitro rooting culture of Catalpa bungei

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