Effects of hormone and light on in vitro preservation of axillary buds of Pinus densiflora resistant to pine wilt disease

QIN Li, WU Xiaoqin*

Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2011, Vol. 35 ›› Issue (05) : 31-34.

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Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2011, Vol. 35 ›› Issue (05) : 31-34. DOI: 10.3969/j.jssn.1000-2006.2011.05.007

Effects of hormone and light on in vitro preservation of axillary buds of Pinus densiflora resistant to pine wilt disease

  • QIN Li, WU Xiaoqin*
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Abstract

In order to preserve axillary buds of the Pinus densiflora with excellent resistance against Bursaphelenchus xylophilus, the effects of different treatments of phytohormones and light on bud preservation were studied. It showed that dark condition was not favorable to in vitro bud preservation at 17 ℃. On the contrary, addition of 1.0 mg/L ABA or 02 mg/L 6BA plus 0.1-0.2 mg/L NAA under lightdark alteration had a positive effect on the bud preservation. In this condition, survival rate of the axillary buds was 94 % or higher and all survived buds proliferated well.

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QIN Li, WU Xiaoqin*. Effects of hormone and light on in vitro preservation of axillary buds of Pinus densiflora resistant to pine wilt disease[J]. Journal of Nanjing Forestry University (Natural Sciences Edition). 2011, 35(05): 31-34 https://doi.org/10.3969/j.jssn.1000-2006.2011.05.007

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