Establishment reaction system of the SRAPPCR in Camellia oleifera

WU Yingying，PENG Fangren*，HAO Mingzhuo，CHEN Longsheng*

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2011, Vol. 35 ›› Issue (05) : 112-116.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2011, Vol. 35 ›› Issue (05) : 112-116. DOI: 10.3969/j.jssn.1000-2006.2011.05.025

Establishment reaction system of the SRAPPCR in Camellia oleifera

  • WU Yingying1，PENG Fangren1*，HAO Mingzhuo1，CHEN Longsheng*
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Abstract

In this paper, orthogonal design to SRAPPCR system for Camellia oleifera cultivar Dabieshan 2 and Ganxing 46 was conducted with the primers combination of Me5 and Em8. Orthogonal designdirect analysis and variance analysis were applied to optimize SRAPPCR amplification system in 4 factors such as Taq DNA polymerase, Mg2+, dNTP and primer. Template DNA was also analyzed by the single element test. The results indicated that an optimal reaction system (20 μL)of SRAPPCR system was established: 120 μmol/min Taq DNA polymerase 125 mmol/L Mg2+, 0.15 mmol/L dNTP, 0.60 μmol/L primer, 60 ng template DNA and 2 μL 10 buffer(Mg2+ free). And, the order of each factor levels affected on the result of SRAPPCR was dNTP, Mg2+, Taq DNA polymerase and primer.

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WU Yingying，PENG Fangren*，HAO Mingzhuo，CHEN Longsheng*. Establishment reaction system of the SRAPPCR in Camellia oleifera[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2011, 35(05): 112-116 https://doi.org/10.3969/j.jssn.1000-2006.2011.05.025

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