JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2011, Vol. 35 ›› Issue (06): 1-5.doi: 10.3969/j.jssn.1000-2006.2011.06.001

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Clone and characterization of ptr-MIR156a promoter in Populus trichocarpa

CHEN Ying,WANG Haoran,XU Qing,HUANG Minren*   

  1. Poplar Germplasm Enhancement & Variety Improvement Lab, Nanjing Forestry University, Nanjing 210037, China
  • Online:2011-11-28 Published:2011-11-28

Abstract: MicroRNAs (miRNAs) are endogenous small RNAs that have large regulatory effects development and stress responses in plants. Recently studies show that the expression of miR156a is intensively related with pathogens infection in Loblolly pine, tobacco, Arabidopsis and maize. To understand the interaction mechanism between miR156a and poplar, we predicted ptr-MIR156a promoter region and amplified three upstreaming fragments of ptr-MIR156a from Populus trichocarpa, 500 bp, 1 000 bp, and 1 500 bp, respectively,analyzed their cisacting elements, then cloned them into plant expression vectors, pMDC83 and transferred into poplar protoplasts. Fluorescence results showed that they drove GFP gene expression at different levels. Based on this result, we could transfer theses fragments into plants and study ptr-miR156a expression patterns and regulation mechanism in the future.

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