Study on extraction and compositions of ginkgolic acids in ginkgo seeds

PAN Hongmei,WU Caie*,CAO Fuliang,FAN Gongjian,YANG Jianting,JIA Shaoqian,LI Tingting

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2011, Vol. 35 ›› Issue (06) : 29-33.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2011, Vol. 35 ›› Issue (06) : 29-33. DOI: 10.3969/j.jssn.1000-2006.2011.06.006

Study on extraction and compositions of ginkgolic acids in ginkgo seeds

  • PAN Hongmei,WU Caie*,CAO Fuliang,FAN Gongjian,YANG Jianting,JIA Shaoqian,LI Tingting
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Abstract

Ginkgolic acids were the allergen in Ginkgo seeds. Based on the results of single factor experiments, the orthogonal experiment were designed to optimize extraction conditions, and the compositions of ginkgolic acids in ginkgo seeds were detected by HPLC. The result indicated that the effect of factors on the extraction was in the order as follows, ethanol concentration, the most important one, and then ratio of material to liquid and extracting time. The optimum conditions defined at last were ethanol concentration 85 %, extraction time 12 h, ratio of material to liquid 1〖DK〗∶12.5. At this conditions, five ginkgolic acid monomers were detected in accordance with standard samples. Verification experiment suggested the extraction yield of ginkgolic acids reached 147.94 μg/g in dry weight, and the residual rate of ginkgolic acids was 2.19 % which was significant lower than the international standard. This method could offer a technological support for the detection of ginkgolic acids and quality control of ginkgo products.

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PAN Hongmei,WU Caie*,CAO Fuliang,FAN Gongjian,YANG Jianting,JIA Shaoqian,LI Tingting. Study on extraction and compositions of ginkgolic acids in ginkgo seeds[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2011, 35(06): 29-33 https://doi.org/10.3969/j.jssn.1000-2006.2011.06.006

References

[1]Victor S, Sierpina M D, Bernd Wollschlaege M D, et al. Ginkgo biloba[J]. American Family Physician, 2003,68(5):923-926.
[2]Zhang Hui, Wang Zhang, Xu Shiying. Optimization of processing parameters for cloudy ginkgo (Ginkgo biloba L.) juice[J]. Journal of Food Engineering, 2007,80:1226-1232.
[3]杨小明,陈钧,钱之玉. 烷基酚酸的生物活性研究进展[J]. 中草药,2003,34(5):附5-6.
[4]方静,谭卫红. 来自银杏提取物的抗肿瘤化合物的研究进展[J]. 生物质化学工程,2008,42(5):56-60.
[5]李中新,孙绪艮. 银杏酚酸及其防治农业害虫研究进展[J]. 山东农业大学学报：自然科学版,2007,38(4):654-656.
[6]庄江兴,邱凌,钟雪,等.银杏酸GA1对酪氨酸酶和黑色素瘤细胞的作用[J].厦门大学学报：自然科学版,2009,48(1):103-106.
[7]姜晓明,赵献军.银杏外种皮提取物对霉菌的抑制作用[J].西北农业学报,2007,16(6):30-33.
[8]杨剑婷,吴彩娥.白果致过敏成分及其致敏机理研究进展[J].食品科技,2009,34(6):282-286.
[9]Helke Hecker, Reiner Johannisson, Egon Koch, et al. In vitro evaluation of the cytotoxic potential of alkylphenols from Ginkgo biloba L.[J]. Toxicology, 2002,177(2/3):167-177.
[10]Koch E, Jaggy H, Chatterjee S S. Evidence for immunotoxic effects of crude Ginkgo biloba L. leaf extracts using popliteal lymph node assay in the mouse[J]. International Journal of Immunopharmacology, 2000, 22(3):229-236.
[11]Ahlemeyer B, Selke D, Schaper C, et al. Ginkgolic acids induce neuronal death and activate protein phosphatase type2C[J]. European Journal of Pharmacology, 2001,430(1):1-7.
[12]Lepoittevin J P, Benezra C, Asakawa Y. Allergic contact dermatitis to Ginkgo biloba L.: relationship with urushiol[J]. Archives of Dermatological Research, 1989,281(4):227-230.
[13]Ponto L L, Schultz S K. Ginkgo biloba extract: review of CNS effects[J]. Annals of Clinical Psychiatry, 2003, 15(2):109-119.
[14]Smith J V, Luo Y. Studies on molecular mechanisms of Ginkgo biloba extract[J]. Applied Microbiology and Biotechnology ,2004, 64(4):465-472.
[15]仰榴青,吴向阳,陈均,等.银杏酸的分光光度法测定[J].分析化学研究报告,2004,32(5):661-664.
[16]吴向阳,仰榴青,陈均.高效液相色谱法测定银杏叶提取物及其制剂中银杏酸的含量[J].药学学报,2003,38(11):846-849.
[17]倪学文,吴谋成.RPHPLC测定银杏外种皮中银杏酚酸的含量[J].食品工业科技,2006(11):174-176.
[18]尹秀莲.银杏外种皮中银杏酸的分离技术研究[D].镇江:江苏大学,2003.
[19]成亮,楼凤昌.银杏外种皮中银杏酚酸的研究概况[J].药学进展,2004,28(5):209-213.
[20]谭卫红.银杏叶烷基酚的化学研究[D].南京:中国林业科学研究院林产化学工业研究所,2001.
[21]仰榴青,吴向阳,陈均.HPLC法测定白果中银杏酚酸的含量[J].药物分析杂志,2004,24(6):636-639.
[22]He Jingren, Xie Bijun. Reversedphase argentation highperformance liquid chromatography in phytochemical analysis of ginkgolic acids in leaves from Ginkgo biloba L.[J]. Journal of Chromatography A, 2002,943(2):303-309.
[23]朱拓,陈国庆,虞锐鹏,等.甲醇溶液吸收光谱与荧光光谱的研究[J].激光技术,2005,29(5):470-472.
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