This research was mainly to discover the origin and development of somatic embryos of Liriodendron hybrids with cellular observation. The results showed that embryogenic callus can be easily induced when used immature zygote embryo as explant. The embryogenic callus structure was tight and compacted and embryogenic cells were small, isodiametric and densely cytoplasmic, with a high nucleoplasmic ratio and small starch grains. Some singlecells from the surface of embryogenic callus can grow into singlecell proembryo with thick cell wall, which show they are independent at the beginning of their lives. The significant character of the somatic embryo was its polarity. Plantlet regenerations were developed from global embryo, heartshaped embryo, torpedoshaped embryo and cotyledon embryo, in which the Vshaped vessel vascular bundles, shoot tip meristem and root tip meristem were observed.
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