The optimum reaction condition of carboxylesterase in poplar was studied using the biochemistry methods. The results indicated that the optimum enzyme dilution factor of carboxylesterase was 10, and the optimum substrate concentration was 3×10-4 mol/L, the optimum pH value was 7.0, and the optimum reaction time was 15 min, and the optimum temperature 30 ℃, for carboxylesterase in poplar. Furthermore, the effect on carboxylesterase from poplar by omethoate, imidacloprid and fipronil was determined. The results showed that carboxylesterase activity of poplar was inhibited by 400,1 600,3 200 and 6 400 mg/mL omethoate, and the inhibition effect of 6 400 mg/mL omethoate was strongest. The carboxylesterase activity of poplar didn’t change, after being treated by 50,100,200,400 and 800 mg/mL imidacloprid for 48 h, and the activity decreased by 400 and 800 mg/mL imidacloprid for 96 h. The carboxylesterase activity of poplar decreased after being treated by 400 mg/mL fipronil for 48 h, and by 800 mg/mL fipronil for 96 h.
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References
[1] 王业霞,史雪岩,梁沛,等.三种内吸性杀虫剂对棉花多酚氧化酶和羧酸酯酶活性的影响[J].农药学学报,2006,8(4):319-322.
[2] Haslam R, Raveton M, Cole D J, et al. The identification and properties of apoplastic carboxylesterases from wheat that catalyse deesterification of herbicides[J]. Pestic Biochem Phys, 2001, 71(3): 178-189.
[3] Youngman R R, Leigh T F, Kerby T A, et al. Pesticides and cotton:effect on photosynthesis, growth, and fruiting[J]. J Econ Entomol, 1990, 83(4): 1549-1557.
[4] Abdel-Reheem S, Belal M H, Gupta G. Photosynthesis inhibition of soybean leaves by insecticides[J]. Environ Pollut, 1991, 74(3): 245-250.
[5] Johnson M W, Welter S C, Toscano N C, et al. Lettuce yield reductions correlated with methyl parathion use[J]. J Econ Entomol, 1983, 76(6): 1390-1394.
[6] 王艳,陈雁君,公茂礼,等.噻虫胺农药胁迫对小白菜过氧化物酶活性的影响[J].中国卫生检验杂志,2008,18(12):2535-2536.
[7] 张清智,陈振德,刘红玉. 氰戊菊酯对小白菜抗氧化酶活性及丙二醛(MDA)含量的影响[J].生态毒理学报,2008,3(5):507-512.
[8] 陈雁君,程晓平,迟亚梅,等. 丙溴磷对油菜过氧化物酶过氧化氢酶活性的影响[J].济宁医学院学报,2007,30(2):88-89.
[9] 张清智,陈振德,王文娇,等.毒死蜱胁迫对小白菜抗氧化酶活性和相关生理指标的影响[J].生态学报,2008,28(9):4524-4530.
[10] Asperen K V. A study of housefly esterases by means of a sensitive colorimetric method[J]. J Insect Physiol, 1962, 8(4): 401-416.
[11] 张龙翔,张庭芳,李令媛.生化试验方法和技术[M].2版.北京:高等教育出版社,1997.
[12] Bradford M M. A rapid and sensitive method for the quantication of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Anal Biochem, 1976, 72: 248-254.
[13] Thompson H M. Esterases as markers of exposure to organophosphates and carbamates [J]. Ecotoxicology, 1999, 8(5): 369-384.
[14] Gupta R C, Dettbarn W D. Role of carboxylesterases in the prevention and potentiation of N-methylcarbamate toxicity [J].Chem-Biol Interact,1993,87(1-3): 295-303.
[15] 曲爱军,郭丽红,孙绪艮,等. 农药胁迫对大叶黄杨SOD和脯氨酸含量的影响[J].农药,2006,45(1):35-37.
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