JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2012, Vol. 36 ›› Issue (05): 37-41.doi: 10.3969/j.jssn.1000-2006.2012.05.006

Previous Articles     Next Articles

Identification and analysis of proteins in the latex Cserum related to the tapping panel dryness in Hevea brasiliensis

ZHOU Xuemei1,2, YANG Lifu1, WANG Zhenhui1, ZOU Zhi1, YUAN Kun1*   

  1. 1. Rubber Research Institute, Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Danzhou 571737, China;
    2. College of Agriculture, Hainan University, Haikou 570100, China
  • Online:2012-09-30 Published:2012-09-30

Abstract: The tapping panel dryness (TPD) in Hevea brasiliensis is a significant limit factor influencing natural rubber production. In this experiment, the twodimensional gel electrophoresis (2DE) was used to study the differentiation of protein expression profiles of latex Cserum between TPD trees and healthy ones of H. brasiliensis. Thirtyone differentially expressed protein spots were obtained by software analysis. After these spots were subjected to matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDITOF MS) followed by searching for NCBInr database, ten spots were successfully identified. Six of them were identified as the unknown proteins and the remaining 4 were the chain A, structure of ubiquitinlike protein, Rub1; β1, 3glucanase; AIR9 protein and flagellar inner dynein arm heavy chain 11. These proteins might play important roles in the process of TPD occuring.

CLC Number: