Composition analysis of the oviposition secretion of Anoplophora glabripennis

doi:10.3969/j.issn.1000-2006.2013.01.014

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2013, Vol. 37 ›› Issue (01): 83-86.doi: 10.3969/j.issn.1000-2006.2013.01.014

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Composition analysis of the oviposition secretion of Anoplophora glabripennis

MA Xiaoqian¹,ZHAO Hongying¹,ZHOU Qi¹,DENG Xun¹,SONG Xiaoshuang¹,YU Wenjing¹,FAN Haijuan²

1.Heilongjiang Academy of Forestry, Harbin 150040,China; 2. College of Forestry, Northeast Forestry University, Harbin 150040,China

Online:2013-02-18 Published:2013-02-18

Abstract

Abstract: To analyze the composition of Anoplophora glabripennis oviposition secretion, A.glabripennis were kept indoors during the spawning period, and oviposition secretion of which was collected. The oven drying method, Bradford method, anthrone colorimetry, Ninhydrin reagent colorimetric method and SDS-PAGE were used to analyze and determinate the fractions of water, protein, soluble sugar and free amino acid content and protein fractions in oviposition secretion. The results showed that oviposition secretion components of A.glabripennis included water(81.98%), protein(4.14%), soluble sugar(0.51%)and free amino acid(0.06%). The molecular weight of main protein components were 107.1 ku,86.7 ku,62.2 ku,47.5 ku and 39.0 ku. The strong spectral bands were 107.1 ku to 86.7 ku and 47.5 ku.

CLC Number:

S763.38

MA Xiaoqian,ZHAO Hongying,ZHOU Qi,DENG Xun,SONG Xiaoshuang,YU Wenjing,FAN Haijuan. Composition analysis of the oviposition secretion of Anoplophora glabripennis[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2013, 37(01): 83-86.

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Composition analysis of the oviposition secretion of Anoplophora glabripennis

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