JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2013, Vol. 37 ›› Issue (02): 50-54.doi: 10.3969/j.issn.1000-2006.2013.02.009

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Selection of wilt-resistant Pinus densiflora superior families for tissue culture propagation

LI Qingqing,YE Jianren*, WU Xiaoqin   

  1. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
  • Online:2013-04-18 Published:2013-04-18

Abstract: Using the embryo seedlings with cotyledons of 27 wilt-resistant Pinus densiflora families as explants, superior families which were good growth vigor, high multiplication rate and strong rooting ability were selected. The average elongation percentages of No.1, 8, 10, 13, 22 and 25 families were up to above 100%; With the increase of proliferation generations, the multiplication coefficient of clustered shoots in each family was variable, but the overall trend was that multiplication coefficient of clustered shoots from generation 1 to generation 2 increased with generation increase, and multiplication coefficient from generation 2 to generation 3 decreased; Adventitious root induction rate of wilt-resistant P. densiflora clones was different, 19-B clone of wilt-resistant P. densiflora was the highest,up to 95.8%.

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