Thermostability of xylanase(XynB)by introducing Arg into its Ser/Thr surface

LI Fei, XIE Jingcong,LI Qi,ZHANG Xuesong,ZHAO Linguo

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (04) : 107-112.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (04) : 107-112. DOI: 10.3969/j.issn.1000-2006.2014.04.020

Thermostability of xylanase(XynB)by introducing Arg into its Ser/Thr surface

  • LI Fei1,2, XIE Jingcong1,LI Qi1,ZHANG Xuesong4,ZHAO Linguo1,3*
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Abstract

Replacing several serine and threonine residues in the Ser/Thr surface of xylanase(XynB)with arginines effectively increased the thermostability of the enzyme by site-directed mutagenesis. The mutated enzymes XynB-104 and XynB-77 were expressed in Pichia pastoris and their enzymatic properties were determined. The optimal temperature of the mutations XynB-104 and XynB-77 were 50 ℃, and the relative activity of mutations were dramatically increased. Both mutated xylanases showed about 80% of maximal activity after incubating in xylan substrate for 2 h at 50 ℃ compared to only 26% activity of the native enzyme. The optimal pH of XynB-104 enzyme had no change, but the pH optimum of the XynB-77 enzyme was increased from 5.0 to 5.5. The results of the mutated enzymes indicated that properly arginines residues introduced into Ser/Thr surface of xylanase XynB might be effective to improve the enzyme thermostability and hydrolysis capacity.

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LI Fei, XIE Jingcong,LI Qi,ZHANG Xuesong,ZHAO Linguo. Thermostability of xylanase(XynB)by introducing Arg into its Ser/Thr surface[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2014, 38(04): 107-112 https://doi.org/10.3969/j.issn.1000-2006.2014.04.020

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