Enzymatic hydrolysis characteristics of xylan in spent liquor of wheat straw alkaline sulfite

HOU Guangbei,FENG Nianjie,ZHANG Lin,DAI Kai,WANG Qi,ZHAI Huamin,XUE Xihua

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (04) : 113-117.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (04) : 113-117. DOI: 10.3969/j.issn.1000-2006.2014.04.021

Enzymatic hydrolysis characteristics of xylan in spent liquor of wheat straw alkaline sulfite

  • HOU Guangbei1,FENG Nianjie1,ZHANG Lin1,DAI Kai1,WANG Qi1,ZHAI Huamin1*,XUE Xihua2
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Abstract

The wheat straw alkaline sulfite pulping(ASP)is hopeful to be a potential non-wood lignocelluloses biomass biorefinery process. However, ASP spent liquor utilization is a bottleneck towards the biorefinery. In the paper, the enzymolysis conditions and properties of three kinds of xylanases from Bacillus alkalophilus, Trichoderma reacei and Pulpzyme H C were investigated based on the relative polysaccharide hydrolysis rate(RPHR). The results indicated that 27.7% of the RPHR was obtained by the xylanase hydrolysis of Bacillus alkalophilus at 6.0 μmol/(mL·min)of enzyme dosage, 50% of spent liquor concentration(weight percentage), pH 7.0, 50 ℃ for 8 h. 48.5% of the RPHR was found by the enzymolysis of Trichoderma reesei at 10.0 μmol/(mL·min)of enzyme dosage, 50% of spent liquor concentration, pH 5.0, 50 ℃ for 8 h. The RPHR of Pulpzyme HC xylanase reached to 48.0% at 8.33 μmol/(mL·min)of enzyme dosage, 50% of spent liquor concentration, pH 8.0, 55 ℃ for 4 h. The reducing sugar content of ASP spent liquor reached to 11.1 g/L from 1.2 g/L by the enzymolysis of Pulpzyme HC. Pulpzyme HC was more powerful and popular comparing with Bacillus alkalophilus and Trichoderma reesei in aspects of the efficiency and industrial application conditions. The enzymolysis of polysaccharide in ASP spent liquor was benefit to the following lignosulphonate separation and utlization.

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HOU Guangbei,FENG Nianjie,ZHANG Lin,DAI Kai,WANG Qi,ZHAI Huamin,XUE Xihua. Enzymatic hydrolysis characteristics of xylan in spent liquor of wheat straw alkaline sulfite[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2014, 38(04): 113-117 https://doi.org/10.3969/j.issn.1000-2006.2014.04.021

References

[1] Hedjazi S, Kordsachia O, Patt R, et al. Alkaline sulfite-anthraquinone(AS/AQ)pulping of wheat straw and totally chlorine free(TCF)bleaching of pulps[J]. Industrial Crops and Products, 2009, 29(1): 27-36.
[2] 龙言泉, 石淑兰, 胡慧仁.草类原料亚硫酸盐法和硫酸盐法制浆中几个问题的研究[J].中国造纸学报, 1986(1): 50-60.Long Y Q, Shi S L, Hu H R. Studies on sulfite and sulfate pulping of certain non-wood fibers[J]. Transactions of China Pulp and Paper, 1986(1): 50-60.
[3] 张琳, 翟华敏.麦草碱性亚硫酸钠制浆生物炼制的研究(I)-深度脱木质素及木质素磺化特性[J]. 林产化学与工业, 2012, 32(1): 43-46.Zhang L, Zhai H M. Study on wheat straw alkaline sulfite pulping biorefinery(I)-characteristics of extended delignification and lignin sulfonation[J]. Chemistry and Industry of Forest Products, 2012, 32(1): 43-46.
[4] 谌尧, 翟华敏. 生物精炼在造纸工业中的应用现状和前景[J]. 中华纸业, 2008(2): 21-23.Chen Y, Zhai H M. The present situation and prospect of the application of biorefinery in the pulp and paper industry[J]. China Pulp and Paper Industry, 2008(2): 21-23.
[5] Andersson A, Persson T, Zacchi G, et al. Comparison of diafiltration and size-exclusion chromatography to recover hemicelluloses from process water from thermomechanical pulping of spruce[J]. Applied Biochem istry Biotechnology, 2007, 137-140(1-12): 971-983.
[6] Ei-Zawawy W K, Ibrahim M M, Abdel-Fattah Y R, et al. Acid and enzyme hydrolysis to convert pretreated lignocellulosic materials into glucose for ethanol production[J]. Carbohydrate Polymers, 2011, 84(3): 865-871.
[7] Bhattacharya P K, Todi R K, Tiwari M, et al. Studies on ultrafiltration of spent sulfite liquor using various membranes for the recovery of lignosulphonates[J]. Desalination, 2005, 174(3): 287-297.
[8] Miller G L. Use of dinitrosalicylic acid reagent for determination of reducing sugar[J]. Analytical Chemistry, 1959, 31(3): 426-428.
[9] 石淑兰, 何福望. 制浆造纸分析与检测[M]. 北京: 中国轻工业出版社, 2010.
[10] Kontturi A K, Sundholm G. The extraction and fractionation of lignosulphonates with long chain aliphatic amines[J]. Acta Chemica Scandinavica: Series A. Physical and Inorganic Chemistry, 1986, 40(2): 121-125.
[11] Vancov T, Mcintosh S. Mild acid pretreatment and enzyme saccharification of sorghum bicolor straw[J]. Applied Energy, 2012, 92: 421-428.
[12] 徐勇, 陈牧, 余世袁, 等. 木聚糖酶水解制取低聚木糖的研究[J]. 林产化学与工业, 2002, 22(2): 57-60.Xu Y, Chen M, Yu S Y, et al. Study on enzymatic hydrolysis of xylan into xylo-oligosaccharide[J]. Chemistry and Industry of Forest Products, 2002, 22(2): 57-60.
[13] 何北海, 林鹿, 孙润仓, 等.木质纤维素化学水解产生可发酵糖研究[J]. 化学进展, 2007, 19(7/8): 1141-1144.He B H, Lin L, Sun R C, et al. Chemical hydrolysis of lignocellulosics into fermentable sugars[J]. Progress in Chemistry, 2007, 19(7/8): 1141-1144.
[14] 毛连山, 勇强, 宋向阳, 等. 超滤分离里氏木霉木聚糖酶的研究[J]. 纤维素科学与技术, 2005, 13(1): 1-3.Mao L S, Yong Q, Song X Y, et al. Studies on ultrafiltration separation of xylanases from Trichoderma reesei Rut C-30[J]. Journal of Cellulose Science and Technology, 2005, 13(1): 1-3.
[15] 王楚, 翟华敏, 刘婧怡, 等. 耐热耐碱木聚糖酶对马尾松KP浆的助漂性能[J]. 南京林业大学学报:自然科学版, 2010, 34(3): 31-34.Wang C, Zhai H M, Liu J Y, et al. Effect of thermostable and alkaline tolerant xylanase pretreatment on the bleaching properties of pine kraft pulp[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2010, 34(3): 31-34.
[16] Hou G B, Dai K, Wu W J, et al. Isolation of lignosulphonate in wheat straw alkaline sulfite pulping spent liquor by combination of xylanase hydrolysis and ultrafiltration [C]//Jin Yongcan. Proceeding of the 4th International Conference on Pulping, Papermaking and Biotechnology. Beijing: China Light Industry Press, 2012: 330-335.
[17] 戴铠, 翟华敏. 耐热耐碱木聚糖酶预处理对麦草浆ECF漂白的影响[J]. 中国造纸学报, 2012, 27(1): 13-17.Dai K, Zhai H M. The effect of high pure thermostable and alkaline tolerant xylanse preatment on ECF bleaching of wheat straw soda-AQ pulp[J]. Transactions of China Pulp and Paper, 2012, 27(1): 13-17.
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