Induction of adventitious roots during tissue culture of Acacia mangium and A. auriculiformis elite trees

HU Feng, SHI Qiong, HUANG Liejian

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (02) : 57-62.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (02) : 57-62. DOI: 10.3969/j.issn.1000-2006.2015.02.010

Induction of adventitious roots during tissue culture of Acacia mangium and A. auriculiformis elite trees

  • HU Feng1, SHI Qiong2, HUANG Liejian2*
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Abstract

Ten years old Acacia mangium and A. auriculiformis elite individual trees were chosen as study materials in this experiment. Proliferated buds were obtained from stem explants being primarily cultured and proliferatively cultured. This study investigated factors related to rooting development of proliferated buds, such as type and concentration of auxins, type of basic medium, concentration of sucrose and active carbon. On the other hand, this experiment also studied the effects of proliferated buds induced by different medium on rooting rate. Results showed that the optimal rooting medium for A. mangium and A. auriculiformis was 1/2MS+IBA 0.5 mg/L+NAA 0.5 mg/L+sucrose 3% and 1/2MS+IBA 0.5 mg/L+NAA 0.25 mg/L+sucrose 3%, respectively. The rooting rate of both species could reach above 93%. Rooting rate of proliferated buds, after being cultured on proliferation medium with low concentration of cytokinin and active carbon, could reach above 90%, and remain stable with the increase of subculture time. Rooting seedlings grow strongly.

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HU Feng, SHI Qiong, HUANG Liejian. Induction of adventitious roots during tissue culture of Acacia mangium and A. auriculiformis elite trees[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(02): 57-62 https://doi.org/10.3969/j.issn.1000-2006.2015.02.010

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