Research on the features of DNA methylation in embryo ripening period ofFraxinus mandshurica Rupr. based on the method of MSAP

doi:10.3969/j.issn.1000-2006.2015.03.008

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (03): 39-44.doi: 10.3969/j.issn.1000-2006.2015.03.008

Previous Articles Next Articles

Research on the features of DNA methylation in embryo ripening period ofFraxinus mandshurica Rupr. based on the method of MSAP

CONG Jianmin¹,CHEN Fengqing¹,SHEN Hailong^2,4*,LI Yuhua^3,4, ZHANG Peng^2,4,YANG Ling^2,4

1.College of life Science, Baicheng Normal College, Baicheng 137000, China;
2.School of Forestry, Northeast Forestry University, Harbin 150040, China;
3.College of Life Sciences, Northeast Forestry University, Harbin 150040, China;
4.State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin 150040, China

Online:2015-05-30 Published:2015-05-30

Abstract

Abstract: To research epigenetic regulation of genome of Fraxinus mandshurica seed development ripening period, Fraxinus mandshurica cotyledons from early July to mid-September 8 stages were selected as test material. Through MSAP(methylation-sensitive amplified polymorphism)method, we detected the change of DNA ethylation in each period. The result indicated that no significant difference among different development stages was detected(in total 933-939 clearly identified strips was detected in each stage). However, the cytosine methylation patterns and variation pattern of CCGG sites showed that, in early July, CNG, CG/CNG loci demethylation sites were higher than other stage, which indicated that large scale of DNA demethylation occurred following seed development of Fraxinus mandshurica. This result could serve as the regulation patterns and as an important basis for providing theoretical basis for seed development.

CLC Number:

Q78
S718

CONG Jianmin,CHEN Fengqing,SHEN Hailong^2,4*,LI Yuhua^3,4, ZHANG Peng^2,4,YANG Ling^2,4. Research on the features of DNA methylation in embryo ripening period ofFraxinus mandshurica Rupr. based on the method of MSAP[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(03): 39-44.

References

[1] Karl M, Ada L, Kerstin M, et al. Regulation of seed germination in the close arabidopsis relative lepidium sativum: A global tissue-specific transcript analysis[J]. Plant Physiol, 2011, 155:1851-1870.
[2] 廖卓毅,钱存梦,马秋月,等.乌桕种子成熟过程中种皮和胚乳超微结构观察[J].南京林业大学学报:自然科学版,2014,38(6):43-47.Liao Z Y,Qian C M,Ma Q Y,et al. Ultrastructure of seed coat and endosperm in the development of Sapium sebiferum seed mature[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2014,38(6):43-47.
[3] 孔冬梅,沈海龙,吕晋慧.水曲柳雌花发育、大孢子发生及胚胎发育的解剖学观察[J].植物研究,2008, 33(7): 387-391.Kong D M, Shen H L, Lü J H. Female flowers development, megaspore and embryonic development of anatomical observation of Fraxinus mandshurica[J]. Journal of Plant Research, 2008, 33(7): 387-391.
[4] Ruiz-García L, Cervera M T, Martínez-Zapater J M. DNA methylation increases throughout Arabidopsis development[J]. Planta, 2005, 222(2): 301-306.
[5] Gehring M, Bubb K L, Henikoff S. Extensive demethylation of repetitive elements during seed development underlies gene imprinting[J]. Science, 2009, 324(5933): 1447-1451.
[6] 陆光远,伍晓明,陈碧云.油菜种子萌发过程中DNA甲基化的MSAP分析[J].科学通报, 2005,50(24):2750-2756.Lu G Y, Wu X M, Chen B Y. Rape seed germination in the process of DNA methylation in the MSAP analysis[J]. ChineseScience Bulletin, 2005, 50(24):2750-2756.
[7] Wu N H. Principle of Gene Engineering[M]. 2nd edition. Beijing: Science Press, 2001:186-188.
[8] Cedar H. DNA methylation and gene activity[J]. Cell, 1988, 53(1):3-4.
[9] Zhang M, Xu C, von Wettstein D, Liu B.Tissue-specific differences in cytosine methylation and their association with differential gene expression in sorghum[J]. Plant Physiol, 2011, 156(4):1955-1966.
[10] Chan S W L, Henderson I R, Jacobsen S E. Gardening the genome: DNA methylation in Arabidopsis thaliana[J]. Nature Reviews Genetics, 2005, 6(5): 351-360.
[11] Lelu M A, Bastien C, Klimaszewska K. An improved method for somatic plantlet production in hybrid larch(Larix×leptoeuropaea):Part Ⅰ.Somatic embryo maturation[J]. Plant Cell Tissue and Organ Culture, 1994, 36(1): 107-115.
[12] Zhu J, Kapoor A, Sridhar V V, et al. The DNA glycosylase/lyase ROS1 functions in pruning DNA methylation patterns in Arabidopsis[J]. Current Biology, 2007, 17(1): 54-59.
[13] Finnegan E J, Peacock W J, Dennis E S. DNA methylation, a key regulator of plant development and other processes[J]. Current Opinion in Genetics & Development, 2000, 10(2):217-223.
[14] Choi Y, Gehring M, Johnson L, et al. DEMETER, a DNA glycosylase domain protein, is required for endosperm gene imprinting and seed viability in Arabidopsis[J]. Cell, 2002, 110(1):33-42.
[15] Xia R, Wang J, Liu C, et al. ROR1/RPA2A, a putative replication protein A2, functions in epigenetic gene silencing and in regulation of meristem development in Arabidopsis[J]. The Plant Cell Online, 2006, 18(1): 85-103.
[16] Rossi V, Motto M, Pellegrin L. Analysis of the methylation pattern of the maize opaque-2(O₂)promoter and in vitro binding studies indicate that the O₂ B-Zip protein and other endosperm factors can bind to methylated target sequences[J]. Journal of Biological Inorganic Chemistry, 1997, 272(21):13758-13765.
[17] Sherman J D, Talbert L E. Vernalization-induced changes of the DNA methylation pattern in winter wheat[J]. Genome, 2002, 45(2): 253-260.
[18] Xiong L Z, Xu C G. Saghai Maro of M A. Patterns of cytosine methylation in an elite rice hybrid and it s parental lines detected by a methylation-ensitive amplification polymorphism technique[J]. Molecular and General Genetics, 1999, 261(2):439-446.
[19] Santos D, Fevereiro P. Loss of DNA methylation affects somatic embryogenesis in Medicago truncatul[J]. Plant Cell, Tissue and Organ Culture, 2002, 70(2): 155-161.
[20] Adams S, Vinkenoog R, Spielman M, et al. Parent-of-origin effects on seed development in Arabidopsis thaliana require DNA methylation[J]. Development, 2000,127(11), 2493-2502.
[21] Xiao W Y, Kendra D. Custard, Roy C, et al. DNA methylation is critical for Arabidopsis embryogenesis and seed viability[J]. The Plant Cell, 2006, 18(4), 805-814.
[22] Le B H, Cheng C, Bui A Q, et al. Global analysis of gene activity during Arabidopsis seed development and identification of seed-specific transcription factors[J]. Plant Biology,2010,107(19):8063-8070.

Related Articles 15

[1]	YANG Yunli, CAO Li, WANG Yang, GU Chenrui, CHEN Kun, LIU Guifeng. Effects of BpGLK1 interference expression on leaf color and growth of Betula pendula ‘Dalecarlica’ [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(1): 18-28.
[2]	WANG Wei, QIU Zhinan, LI Shuang, BAI Xiangdong, LIU Guifeng, JIANG Jing. CRISPR/Cas9 ribonucleoprotein-mediated precise mutation of BpGLK1 in birch without T-DNA insertion [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(1): 11-17.
[3]	WANG Zhipu, LI Zhuorong, LUO Zhibin, DENG Shurong. Mechanisms of PagAPY1 in regulating drought tolerance in Populus alba × P. glandulosa [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(6): 105-112.
[4]	QUE Feng, LIU Qingnan, ZHA Ruofei, WEI Qiang. Identification and analysis of senescence related WRKYs in sheath of Bambusa multiplex [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(6): 113-123.
[5]	LIU Yamei, LIU Shengquan, ZHOU Liang, HU Jianjun, ZHAO Zicheng, ZHENG Xiangli. Anatomical characteristics and radial variations in eight poplar clones/cultivars [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(1): 234-240.
[6]	QUE Feng, ZHA Ruofei, WEI Qiang. Advances in research of cellulose synthase genes in plants [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(6): 207-214.
[7]	GONG Jue, BI Hao, YANG Wenlu, WANG Dongming, WANG Lehui, JIANG Yuanzhong, MA Tao. Characterization of IpSAD gene family in Idesia polycarpa and functional identification [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(6): 261-270.
[8]	HE Qingqing, LIU Chuanqiang, LI Jianjian, WANG Jingjing, YAO Xiang, ZHOU Shenghao, CHEN Ying, WANG Haoran. Cloning of EoNLA gene in Eremochloa ophiuroides and the transgenic Arabidopsis phenotypic characterization under various phosphorus levels [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(3): 134-142.
[9]	WANG Zhanjun, WU Ziqi, WANG Zhaoxia, OU Zulan, LI Jie, CAI Qianwen, XU Zhongdong, ZHANG Zhaoliang. A comparative study of the evolution and codon usage bias in WOX gene family of three Camellia sinensis cultivars [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(2): 71-80.
[10]	HE Xudong, ZHENG Jiwei, JIAO Zhongyi, DOU Quanqin, HUANG Libin. Genetic diversity and structure analyses of Quercus shumardii populations based on SLAF-seq technology [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(2): 81-87.
[11]	YAN Zhixiang, YANG Haiyan, FAN Sufan, WU Wenlong, LYU Lianfei, LI Weilin. Analysis of the expression of sucrose phosphate synthase genes during the development of blackberry fruit [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(1): 179-186.
[12]	ZHANG Qun, JI Xiaoyu, HE Zihang, WANG Zhibo, TIAN Zengzhi, WANG Chao. Cloning and salt tolerance analysis of BpGRAS1 gene in Betula platyphylla [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(5): 38-46.
[13]	ZHOU Fangwei, WU Huaitong, YIN Tongming. Characteristics of MIXTA/MIXTA-like genes and their functions in regulating plant epidermal cells differentiation [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(4): 229-237.
[14]	JU Ye, JIANG Jianping, YIN Zengfang, WEI Qiang. Full-length transcriptome sequencing and annotation analyses of Bambusa multiplex sheath [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(6): 175-183.
[15]	CHEN Wenwen, WU Huaitong, CHEN Yingnan. Gene duplications and functional divergence analyses of the SPL gene family [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(5): 55-66.

Metrics

Comments

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

Research on the features of DNA methylation in embryo ripening period ofFraxinus mandshurica Rupr. based on the method of MSAP

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Author

Reader

Reviewer