Abstract: NAC transcription factors are the key transcription regulatory factors which involved in plant multiple biological functions, such as plant development, hormones regulation, and environmental stimuli. The full length cDNA sequence of a NAC-like gene from the hybrid of Populus deltoides × P. euramericana cv ‘Nanlin895’, was cloned by RT-PCR and RACE methods, named PdNAC1, which contains an ORF of 906 bp and encodes 302 amino acids with the molecular weight of about 34.23 ku ond pI of 7.44. The putative protein sequence analysis showed that PdNAC1contained conserved NAC domain including five sub-domains A, B, C, D, and E which can bind DNA. Meanwhile, the C-terminal of PdNAC1 is highly different and that is very typical in NAC transcription factors. Furthermore, the results of sub-cellular localization in poplar protoplast showed that the PdNAC1 transcription factor is mainly located in the cell nucleus, but also translocated on the cell membrane. However,further investigation would be needed to reveal whether the PdNAC1 is corresponding to transmembrane domain.