Effects of carbon source and nitrogen source on solid state fermentation for the production of β-mannanase

ZHANG Xiaoyan, LI Xin, WANG Jing, YU Shiyuan, YONG Qiang

Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2015, Vol. 39 ›› Issue (04) : 143-148.

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Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2015, Vol. 39 ›› Issue (04) : 143-148. DOI: 10.3969/j.issn.1000-2006.2015.04.025

Effects of carbon source and nitrogen source on solid state fermentation for the production of β-mannanase

  • ZHANG Xiaoyan, LI Xin, WANG Jing, YU Shiyuan, YONG Qiang*
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Abstract

The residues from xylo-oligosaccharides production was applied for β-mannanase preparation instead of wheat bran, and effects of different kinds and ratio of carbon sources, inducer, nitrogen sources and loading on solid state fermentation for β-mannanase preparation by Aspergillus niger were investigated in this work. Residues of xylo-oligosaccharides production could be instead of wheat bran partly for β-mannanase preparation. Solid state fermentation of residues of xylo-oligosaccharides production and bran by A. niger for five days, which the mass ratio of residues of xylo-oligosaccharides production to wheat bran was 4:6, 2% of ammonia sulfate and 3% of konjac flour loading in the medium, the β-mannanase activity and β-mannosidase activity were 341.8 and 7.02 μmol/(min·g), respectively, and soluble protein content was 8.40 mg/g.

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ZHANG Xiaoyan, LI Xin, WANG Jing, YU Shiyuan, YONG Qiang. Effects of carbon source and nitrogen source on solid state fermentation for the production of β-mannanase[J]. Journal of Nanjing Forestry University (Natural Sciences Edition). 2015, 39(04): 143-148 https://doi.org/10.3969/j.issn.1000-2006.2015.04.025

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