Effects of NaCl treatment on the growth, salt tolerance and flavonoids 
 accumulation in Ginkgo biloba suspension cells

doi:10.3969/j.issn.1000-2006.2015.06.009

Effects of NaCl treatment on the growth, salt tolerance and flavonoids accumulation in Ginkgo biloba suspension cells

CHEN Ying, LUO Yongya, QIU Nafei, WANG Ruiqi, SHENG Lili, CAO Fuliang

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (06) : 45-50.

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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (06) : 45-50. DOI: 10.3969/j.issn.1000-2006.2015.06.009

Effects of NaCl treatment on the growth, salt tolerance and flavonoids accumulation in Ginkgo biloba suspension cells

CHEN Ying^1,2, LUO Yongya^1,2, QIU Nafei^1,2, WANG Ruiqi^1,2, SHENG Lili^1,2, CAO Fuliang^1,3

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Abstract

The salt tolerance ability, cell growth and flavonoids accumulation in Ginkgo biloba L. suspension cells were investigated by adding 0, 50, 100, 150 mmol/L NaCl into the MS liquid culture medium. The results showed that the cell growth increased with the increasing of both salt treatment time and concentration in the early treatment(4-8 days), while decreased during 12-20 days under NaCl treatments. Moderate and high dose of salinity(100-150 mmol/L)had a certain tolerance to salt stimulation by increasing the soluble protein content at early NaCl treatment(4-8 days)compared to the control. The growth biomass was reduced by the increase of salinity dose during 8-16 days, whereas the high level of proline and flavonoids was found and it suggested that the Ginkgo biloba cells could enhance the osmotic adjustment capacity and oxidation resistance ability by stimulating the biosynthesis of proline and flavonoids at this period. Furthermore, the intensity of salt stress was higher than the cell self-regulation capacity, and serious damage and brown appeared in cells at the 20^th day. The growth of suspension cells was not obviously restrained under 50 mmol/L NaCl treatment compared to the control, due to the low level of relative electric conductivity(REC)and MDA content in cell observed. Furthermore, the PAL activity increased significantly at the 8^th day and the highest flavonoid accumulation and total flavonoids yields(129.0 mg/L)were found at 12^th day. The flavonoids content at 100 mmol/L NaCl treatment increased by 29.0% than the control, while the total flavonoids yield(98.4 mg/ L)was only 10.9% higher than the control. However, the flavonoids content at 150 mmol/L NaCl could maintain the high level at 8^th day and it was 66.3% times higher than control, meanwhile the total flavonoids yield was(104.0 mg /L)attained at 8^th day, too. Therefore, the cell sampled time at 12^th day and at 8^th day was beneficial to flavonoids production under 50 mmol/L and 150 mmol/L NaCl treatment, respectively.

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CHEN Ying, LUO Yongya, QIU Nafei, WANG Ruiqi, SHENG Lili, CAO Fuliang. Effects of NaCl treatment on the growth, salt tolerance and flavonoids accumulation in Ginkgo biloba suspension cells[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(06): 45-50 https://doi.org/10.3969/j.issn.1000-2006.2015.06.009

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