Relationship between the meiosis processes of microsporocytes and morphology 
of male flower buds and anthers in Populus deltoides Marsh

doi:10.3969/j.issn.1000-2006.2016.02.008

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 40 ›› Issue (02): 48-52.doi: 10.3969/j.issn.1000-2006.2016.02.008

Previous Articles Next Articles

Relationship between the meiosis processes of microsporocytes and morphology of male flower buds and anthers in Populus deltoides Marsh

XIN Haoyang, WANG Shuai, LIU Guangxin, ZHEN Yan, SHI Jisen, KUANG Hualin, XI Mengli^*

Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

Online:2016-04-18 Published:2016-04-18

Abstract

Abstract: With carbol fuchsin staining, we studied the relationship between the meiosis processes of microsporocytes and the morphology of male flower buds and anthers in Populus deltoides Marsh, laying the foundations for inducing 2n gametes in this species. The results were as follows: The meiosis ended before the catkin came out of the bud scale. When the bud scale from the top or lateral of the flower bud began to loosen, all the meiosis processes were found from the same flower bud, and this time was optimum for inducing 2n gametes. The length of anthers in P. deltoides Marsh and the stages of meiosis were closely related. The 0.5 mm-long anther was at the stage of microsporocytes, the 1.0 mm-long anther was at the stage of leptotene, pachytene, metaphase Ⅰ, telophase Ⅰ and dyad, the 1.5 mm-long anther was at the stage of tetrad, the 2.0 mm-long anther was at the stage of pollen. Different flower buds in one flowering branch or different florets in one flower bud showed non-synchronization in the meiosis processes of microsporocytes, but different anthers in one floret showed synchronization in the processes. The behavior of chromosomes was standard during the meiosis processes of microsporocytes in P. deltoides Marsh. It could make this species a good experimental material for molecular cytogenetic study.

CLC Number:

S718

XIN Haoyang, WANG Shuai, LIU Guangxin, ZHEN Yan, SHI Jisen, KUANG Hualin, XI Mengli. Relationship between the meiosis processes of microsporocytes and morphology of male flower buds and anthers in Populus deltoides Marsh[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2016, 40(02): 48-52.

References

[1] 方升佐,徐锡增,吕士行. 杨树定向培育[M].合肥:安徽科学技术出版社, 2004.
[2] 罗建勋,索铁阿罗,辜云杰,等. 滇杨与美洲黑杨派间杂交育种试验初报[J]. 四川林业科技,2010, 31(2): 57-59.Doi:10.3969/j.issn.1003-5508.2010.02.012. Luo J X,Suotie A L,Gu Y J,et al. A preliminary study of intersectional hybridization between Populus yunnanensis and Populus deltoides [J]. Journal of Sichuan Forestry Science and Technology, 2010, 31(2): 57-59.
[3] 李云. 杨树三倍体选育研究进展[J]. 植物学通报, 2001, 18(4): 452-458.Doi:10.3969/j.issn.1674-3466.2001.04.009. Li Y. Advances in studies of triploid poplar breeding[J]. Chinese Bulletin of Botany, 2001, 18(4): 452-458.
[4] Ramanna M S, Jacobsen E. Relevance of sexual polyploidization for crop improvement—a review [J]. Euphytica, 2003, 133: 3-8.Doi: 10.1023/A:1025600824483.
[5] Van Tuyl J M, Lim K B. Interspecific hybrization and polyploidisation as tools in ornamental plant breeding[J].Acta Horticulturae, 2003, 612: 13-22.Doi:10.17660/ActaHortic.2003.612.1.
[6] 张勇,张守攻,齐力旺,等. 杨树——林木基因组学研究的模式物种[J]. 植物学通报,2006, 23(3): 286-293.Doi:10.3969/j.issn.1674-3466.2006.03.009. Zhang Y,Zhang S G,Qi L W,et al. Poplar as a model for forest tree in genome research [J]. Chinese Bulletin of Botany, 2006, 23(3): 286-293.
[7] 康向阳.杨树染色体数目和形态观察[J].甘肃农业大学学报, 1996,31(1):67-70. Kang X Y. Chromosome count and shape of poplar[J]. Journal of Gansu Agricultural University,1996,31(1):67-70.
[8] Hans de Jong J, Fransz P, Zabel P. High resolution FISH in plants—techniques and applications[J]. Trends of Plant Science, 1999, 4(7):258-263.Doi:10.1016/S1360-1385(99)01436-3.
[9] 许雯婷,赵健,赵楠,等. 欧洲黑杨花粉母细胞减数分裂观察及其不同步性分析[J]. 西北植物学报,2011,31(10):1981-1987. Xu W T,Zhao J,Zhao N,et al. Meiosis of pollen mother cells and their asynchrony in Populus nigra L. [J]. Acta Bot Boreal-Occident Sin,2011,31(10):1981-1987.
[10] 许重阳,王君,叶粟,等. 白杨杂种三倍体小孢子母细胞减数分裂及花粉形态观察[J]. 西北植物学报,2011,31(12):2454-2458. Xu C Y,Wang J,Ye S,et al. Meiosis of microsporocyte and variation of pollen in an allotriploid white poplar[J]. Acta Bot Boreal-Occident Sin,2011,31(12):2454-2458.
[11] 唐湫钥,王君,刘明虎,等. 小胡杨花粉母细胞减数分裂染色体行为研究[J]. 西北植物学报,2010,30(7):1351-1358. Tang Q Y,Wang J,Liu M H,et al. Chromosome behavior of meiosis in pollen mother cell of Populus simonii ×P. euphratica[J]. Acta Bot Boreal-Occident Sin,2010,30(7):1351-1358.
[12] 祁传磊,李开隆,肖静,等. 大青杨花粉母细胞减数分裂及其发育[J]. 东北林业大学学报,2009,37(3):72-75.Doi:10.3969/j.issn.1000-5382.2009.03.027. Qi C L,Li K L,Xiao J,et al. Meiosis of pollen mother cells and pollen development of Populus ussuriensis[J]. Journal of Northeast Forestry University,2009,37(3):72-75.
[13] 王君, 康向阳, 李代丽, 等. 通辽杨花粉母细胞减数分裂及其染色体行为研究[J]. 西北植物学报, 2006, 26(11): 2231-2238.Doi:10.3321/j.issn:1000-4025.2006.11.007. Wang J,Kang X Y,Li D L,et al. Meiosis and chromosome behavior of pollen mother cell in Populus simonii Carr.×P. nigra L. ‘Tongliao'[J]. Acta Bot Boreal-Occident Sin, 2006, 26(11): 2231-2238
[14] 李艳华, 马洁, 康向阳, 等. 毛白杨大孢子母细胞减数分裂进程及其即时判别[J]. 北京林业大学学报, 2005, 27(2): 70-74.Doi:10.3321/j.issn.1000-1522.2005.02.014. Li Y H, Ma J,Kang X Y,et al. Stages of MMC meiosis and its timely discrimination of white poplars[J]. Journal of Beijing Forestry University,2005, 27(2): 70-74.
[15] 康向阳, 朱之悌, 张志毅. 毛白杨花粉母细胞减数分裂及其进程的研究[J]. 北京林业大学学报, 2000, 22(6): 5-7.Doi:10.3321/j.issn:1000-1522.2000.06.002. Kang X Y,Zhu Z T,Zhang Z Y. Meiosis and its stages of pollen mother cells in Chinese white poplar[J]. Journal of Beijing Forestry University,2000, 22(6): 5-7.
[16] 赵晨光,李燕杰,张平冬,等.胡杨未减数2n花粉形成的细胞学机制[J].南京林业大学学报(自然科学版),2015,39(6):40-44.Doi:10.3969/j.issn.1000-2006.2015.06.008. Zhao C G, Li Y J, Zhang P D, et al. Gytological mechanism of unreduced 2n pollen formation in Populus euphratica[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2015,39(6):40-44.
[17] 康向阳, 朱之悌, 张志毅. 毛白杨异源三倍体形态和减数分裂观察[J].北京林业大学学报, 1999, 21(1):1-5.Doi:10.3321/j.issn:1000-1522.1999.01.002. Kang X Y,Zhu Z T,Zhang Z Y. Morphology and meiosis of Chinese white poplar[J]. Journal of Beijing Forestry University,1999, 21(1):1-5.
[18] 侯延侠, 康向阳. 响叶杨大、小孢子母细胞减数分裂进程及其对应关系[J]. 西北植物学报, 2006, 26(1): 46-49.Doi:10.3321/j.issn:1000-4025.2006.01.009. Hou Y X,Kang X Y. Meiosis stages and their corresponding relation of Populus adenopoda megasporocytes and microsporocytes [J]. Acta Bot Boreal-Occident Sin, 2006, 26(1): 46-49.
[19] 鲁敏,王君,王旭军,等. 响叶杨小孢子母细胞减数分裂及染色体行为的研究[J].植物科学学报,2011,29(2):171-177.Doi:10.3724/SP.J.1142.2011.20171. Lu M,Wang J,Wang X J,et al. Meiosis and chromosome behavior of microsporocytes in Populus adenopoda Maxim.[J]. Plant Science Journal,2011,29(2):171-177.
[20] 郭军洋,陈劲枫,钱春桃,等. 植物减数分裂染色体配对与染色体组分析的研究进展[J]. 植物学通报, 2004, 21(5): 513-520.Doi:10.3969/J.issn.1674-3466.2004.05.001. Guo J Y,Chen J F,Qian C T,et al. Meiotic chromosome pairing research and genome analysis in plants [J]. Chinese Bulletin of Botany, 2004, 21(5): 513-520.

Related Articles 15

[1]	YANG Yong. Systematic classification of gymnosperms: past, present and future [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 14-26.
[2]	ZHANG Rui, ZHOU Zhenghu, WANG Chuankuan, JIN Ying. Xylem anatomical and hydraulic traits of trees with different wood properties in a temperate forest in northeast China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 229-236.
[3]	HUANG Yongjian, XUN Hang, ZHANG Bao, YOU Junhao, YAO Xi, TANG Feng. Simultaneous determination of eight phenolic acids in bamboo shoots by HPLC and its applications [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 237-244.
[4]	DENG Yunfei. Review on the systematics of the family Styracaceae [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 27-35.
[5]	LI Jialiang, WU Dayu, MAO Kangshan. Taxonomy and diversity of the genus Cupressus: current status and recommendations [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 36-45.
[6]	LI Yongfu, YANG Qinghua, CHEN Lin, ZHANG Min, XIANG Qibai, WANG Xianrong, DUAN Yifan. An infrageneric taxonomic revision of the genus Osmanthus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 58-62.
[7]	YANG Hao, LIU Chao, ZHUANG Jiayao, ZHANG Shutong, ZHANG Wentao, MAO Guohao. Effects of different carrier bacterial fertilizers on growth, photosynthetic characteristics and soil nutrients of Amorpha fruticosa [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 81-89.
[8]	DING Yong, LIU Xin, ZHANG Jinchi, WANG Yuhao, CHEN Meiling, LI Tao, LIU Xiaowu, ZHOU Yuexiang, SUN Lianhao, LIAO Yi. Effects of acid rain-based transformation on Cunninghamia lanceolata fine root growth and soil nutrient content [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 90-98.
[9]	WU Yan, HUANG Qing, LIU Xun, ZHENG Rui, CEN Jiabao, DING Bo, ZHANG Yunlin, FU Yuhong. Effects of Pinus massoniana plantation age on soil physical and chemical properties in Karst areas in southwest China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 99-107.
[10]	BU Xiaoting, FU Wei, LI Shuxian, XU Zhibiao, PENG Daqing, XU Linqiao. Effects of rejuvenation and hormone treatment on the rooting of softwood cuttings of Quercus texana and anatomical observations of rooting [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 129-136.
[11]	DU Jincheng, LI Xinxin, WANG Zeliang, LIU Si, ZHONG Yi, WANG Lihua. Response of physiological indexes of three Olea europaea cultivars to PEG stress [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 137-143.
[12]	FANG Jing, ZHANG Shuman, YAN Shanchun, WU Shuai, ZHAO Jiaqi, MENG Zhaojun. Effects of the compound inoculation of two arbuscular mycorrhizal(AM) fungi on the resistance of Populus pseudo-cathayana × P. deltoides leaves to Hyphantria cunea [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 144-154.
[13]	ZHANG Xinfang, WANG Guangpeng, ZHANG Shuhang, LI Ying, GUO Yan. Screening and analysis of differential secondary metabolites in Castanea mollissima with different levels of resistance to Oligonychus ununguis [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 234-240.
[14]	YANG Hong, YI Xiangui, WANG Xianrong, WU Tong, ZHOU Huajin, CHEN Jie, LI Meng, ZHU Zhaoqing. Prunus discoidea‘Yuanchun’:a new cultivar of cherry blossom [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 275-276.
[15]	TIAN Mengyang, ZHU Shulin, DOU Quanqin, JI Yanhong. The effects of intercropping of Carya illinoinensis and Camellia sinensis ‘Anjibaicha’ on photosynthetic characteristics of C. sinensis tree during rapid growth period [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 86-96.

Metrics

Comments

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

Relationship between the meiosis processes of microsporocytes and morphology of male flower buds and anthers in Populus deltoides Marsh

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Author

Reader

Reviewer