Bioinformation and expression pattern analysis of FmGT8 gene family in Fraxinus mandshurica

ZHOU Shan, ZHAN Yaguang, DONG Heng, LIU Jingjing, ZENG Fansuo

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2016, Vol. 40 ›› Issue (02) : 27-32.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2016, Vol. 40 ›› Issue (02) : 27-32. DOI: 10.3969/j.issn.1000-2006.2016.02.005

Bioinformation and expression pattern analysis of FmGT8 gene family in Fraxinus mandshurica

  • ZHOU Shan, ZHAN Yaguang, DONG Heng, LIU Jingjing, ZENG Fansuo*
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Abstract

The structure and characteristics of glycosyltransferase 8 family proteins in Fraxinus mandshurica were studied in this paper. The spatio temporal expression patterns and the response mechanism to gravity were revealed. On the one hand, analysis of structure and temporal expression patterns of FmGT8 genes provide the basis and inspiration for other woody plants; on the other hand, the study of gene function FmGT8 lay the foundation for directing the transformation of the cell wall composition and wood features and the use of biomass energy. Four FmGT8 genes were cloned from Fraxinus mandshurica in previous research. Application of bioinformation software on FmGT8 and its encoded protein was analyzed in detail. Ash leaf, xylem, skin samples were selected from May to September. The expression pattern in the circadian variation of FmGT8 genes was analyzed by real-time PCR. Tension wood of ash was sampled by one day, three days and seven days treatment. Analysis of the response patterns of FmGT8 gene transcription to gravity treatment. Three full-length FmGT8 genes(FmGT8-1, FmGT8-2, FmGT8-3)and a FmGT8 gene fragment(FmGT8-4)were obtained, which were uploaded to the NCBI and got the accession number(KP307672, KP307673, KP307674 and KP307675). The expressions of FmGT8-1, FmGT8-2, FmGT8-3 were the highest in xylem, while FmGT8-4 in the skin. The expression of FmGT8-1, FmGT8-2, FmGT8-3 were the highest in June, however, FmGT8-4 expressed in September was highest. The expression patterns of FmGT8 family genes varied in the response to gravity treatment. The expression levels of FmGT8-1, FmGT8-2. FmGT8-4 were the highest in TW, whereas FmGT8-3 in the OW. Expression patterns of FmGT8 family genes were difference which showed that the expressions of FmGT8 genes were spatiotemporal specificity. There were differences among the four genes in response to gravity treatment, which suggested FmGT8 gene family might be involved in cell wall and lignin synthesis.

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ZHOU Shan, ZHAN Yaguang, DONG Heng, LIU Jingjing, ZENG Fansuo. Bioinformation and expression pattern analysis of FmGT8 gene family in Fraxinus mandshurica[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2016, 40(02): 27-32 https://doi.org/10.3969/j.issn.1000-2006.2016.02.005

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