Analysis of the differential proteins in the graft healing process of pecan(Carya illinoinensis)

MO Zhenghai, HE Haiyang, CHEN Wenjing, DENG Qiuju, PENG Fangren

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2017, Vol. 41 ›› Issue (06) : 19-25.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2017, Vol. 41 ›› Issue (06) : 19-25. DOI: 10.3969/j.issn.1000-2006.201703021

Analysis of the differential proteins in the graft healing process of pecan(Carya illinoinensis)

  • MO Zhenghai, HE Haiyang, CHEN Wenjing, DENG Qiuju, PENG Fangren*
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Abstract

【Objective】Grafting is an important method for propagating pecan. This paper aimed to reveal the mechanism underlying graft healing on the proteomic level. 【Method】 We investigated the differential proteins in the graft union of pecans at four developmental stages(1, 6, 10 and 25 days after grafting)based on two-dimensional gel electrophoresis and MALDI-TOF/TOF-MS. 【Result】 In total, 48 differential proteins were identified, and these identified proteins were classified into seven functional categories: energy metabolism, stress and defense responses, cell growth, secondary metabolism, protein synthesis, amino acid metabolism and function unknown. 【Conclusion】Fructose-bisphosphate aldolase, phosphoglycerate kinase, pyruvate decarboxylase and adenosine triphosphatase could provide adequate energy for the graft healing of pecan. Ascorbate peroxidase and peroxiredoxins can eliminate the excessive reactive oxygen species caused by grafting. Soluble inorganic pyrophosphatase may play a role in promoting callus proliferation. Metacaspase and alpha tubulin may be associated with tracheary element differentiation.

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MO Zhenghai, HE Haiyang, CHEN Wenjing, DENG Qiuju, PENG Fangren. Analysis of the differential proteins in the graft healing process of pecan(Carya illinoinensis)[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2017, 41(06): 19-25 https://doi.org/10.3969/j.issn.1000-2006.201703021

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