Effects of flooding stress on the growth and chlorophyll fluorescence kinetics of Malus hupehensis

ZHANG Hu, CAO Fuliang, FAN Junjun, ZHANG Wangxiang

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2018, Vol. 42 ›› Issue (01) : 35-40.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2018, Vol. 42 ›› Issue (01) : 35-40. DOI: 10.3969/j.issn.1000-2006.201703065

Effects of flooding stress on the growth and chlorophyll fluorescence kinetics of Malus hupehensis

  • ZHANG Hu1, CAO Fuliang2*, FAN Junjun2, ZHANG Wangxiang2
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Abstract

【Objective】The goal of this study was to evaluate waterlogging tolerance of Malus hupehensis, and analyze the waterlogging resistance in terms of pigment relative content and chlorophyll fluorescence kinetics. 【Method】The growth, morphology, leaf appearance, and chlorophyll fluorescence characteristics of M. hupehensis during flooding were analyzed by artificial simulation of flooding stress.【Result】①The growth of M. hupehensis was significantly inhibited by flooding treatment, but the survival rate of seedlings was 100% after 30 days to 200 days flooding. ②The leaves gradually turned red, and the ratio of relative content of chlorophyll to carotenoids and anthocyanin(mChl/mCar+Anth)decreased significantly after flooding. ③ The results of chlorophyll fluorescence kinetics showed that the initial fluorescence Fo(at the O phase)was significantly higher than that in the control, but the maximum fluorescence Fm(at the P phase)was significantly lower than that in the control. After the flooding, the increasing rate of the variable fluorescence Vj(3.4%-9.0%)compared with that of the control was larger than that of Vk(3.8%-11.6%). With prolongation of flooding stress, the number of reaction centers(RC/CSm)and the capacity of PSⅡ(Sm)significantly decreased, and the transfer of electrons from QA to QB was inhibited, which resulted in reducing the ability of electronic transmission from PSⅡ to PSⅠ(Ψo and φRo). Finally, as a result, the performance indexes(PItotal)significantly decreased. However, the value of DIo/CSm increased to reduce the generation of excitation energy.【Conclusion】Flooding significantly inhibited the growth of M. hupehensis, but no seedling died. Therefore, it could be considered that M. hupehensis had a certain waterlogging resistance. Although the effect on the electron acceptor of PSⅡwas greater than that of the donor, M. hupehensis could decrease the excitation energy to improve the flood resistance by increasing heat dissipation ratio, carotenoid and anthocyanin relative content.

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ZHANG Hu, CAO Fuliang, FAN Junjun, ZHANG Wangxiang. Effects of flooding stress on the growth and chlorophyll fluorescence kinetics of Malus hupehensis[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2018, 42(01): 35-40 https://doi.org/10.3969/j.issn.1000-2006.201703065

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