Effects of flooding stress on the growth and chlorophyll fluorescence 
kinetics of Malus hupehensis

doi:10.3969/j.issn.1000-2006.201703065

Effects of flooding stress on the growth and chlorophyll fluorescence kinetics of Malus hupehensis

ZHANG Hu, CAO Fuliang, FAN Junjun, ZHANG Wangxiang

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2018, Vol. 42 ›› Issue (01) : 35-40.

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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2018, Vol. 42 ›› Issue (01) : 35-40. DOI: 10.3969/j.issn.1000-2006.201703065

Effects of flooding stress on the growth and chlorophyll fluorescence kinetics of Malus hupehensis

ZHANG Hu¹, CAO Fuliang^2*, FAN Junjun², ZHANG Wangxiang²

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Abstract

【Objective】The goal of this study was to evaluate waterlogging tolerance of Malus hupehensis, and analyze the waterlogging resistance in terms of pigment relative content and chlorophyll fluorescence kinetics. 【Method】The growth, morphology, leaf appearance, and chlorophyll fluorescence characteristics of M. hupehensis during flooding were analyzed by artificial simulation of flooding stress.【Result】①The growth of M. hupehensis was significantly inhibited by flooding treatment, but the survival rate of seedlings was 100% after 30 days to 200 days flooding. ②The leaves gradually turned red, and the ratio of relative content of chlorophyll to carotenoids and anthocyanin(m_Chl/m_Car+Anth)decreased significantly after flooding. ③ The results of chlorophyll fluorescence kinetics showed that the initial fluorescence F_o(at the O phase)was significantly higher than that in the control, but the maximum fluorescence F_m(at the P phase)was significantly lower than that in the control. After the flooding, the increasing rate of the variable fluorescence V_j(3.4%-9.0%)compared with that of the control was larger than that of V_k(3.8%-11.6%). With prolongation of flooding stress, the number of reaction centers(RC/CS_m)and the capacity of PSⅡ(S_m)significantly decreased, and the transfer of electrons from Q_A to Q_B was inhibited, which resulted in reducing the ability of electronic transmission from PSⅡ to PSⅠ(Ψ_o and φ_Ro). Finally, as a result, the performance indexes(PI_total)significantly decreased. However, the value of DI_o/CS_m increased to reduce the generation of excitation energy.【Conclusion】Flooding significantly inhibited the growth of M. hupehensis, but no seedling died. Therefore, it could be considered that M. hupehensis had a certain waterlogging resistance. Although the effect on the electron acceptor of PSⅡwas greater than that of the donor, M. hupehensis could decrease the excitation energy to improve the flood resistance by increasing heat dissipation ratio, carotenoid and anthocyanin relative content.

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ZHANG Hu, CAO Fuliang, FAN Junjun, ZHANG Wangxiang. Effects of flooding stress on the growth and chlorophyll fluorescence kinetics of Malus hupehensis[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2018, 42(01): 35-40 https://doi.org/10.3969/j.issn.1000-2006.201703065

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