The karyotype and FISH analysis of 45S and 5S rDNA on the 
chromosomes of Phyllostachys edulis and Phyllostachys edulis ‘Pachyloen'

doi:10.3969/j.issn.1000-2006.201802013

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (02): 203-208.doi: 10.3969/j.issn.1000-2006.201802013

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The karyotype and FISH analysis of 45S and 5S rDNA on the chromosomes of Phyllostachys edulis and Phyllostachys edulis ‘Pachyloen'

HAN Yanli¹,GUO Qirong^2*, HAN Yonghua^1*, YU Zhaoyuan,MI Yueyin

(1. Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences,Jiangsu Normal University, Xuzhou 221116, China; 2. Co-innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China)

Online:2019-03-30 Published:2019-03-30

Abstract

Abstract: 【Objective】Phyllostachys edulis ‘Pachyloen' is a cultivar of Phyllostachys edulis, which is an economically-important germplasm. Cytogenetic research on this germplasm provide a cytomolecular basis for genetic research on bamboo species.【Method】Karyotype analysis and physical localization of 45S and 5S rDNA on the chromosomes of Ph. edulis and Ph. edulis ‘Pachyloen' were performed by double color fluorescence in situ hybridization(FISH).【Result】The results of karyotype analysis indicated that the chromosome number in the two materials was 2n=48, the karyotype formula of Ph. edulis and Ph. edulis ‘Pachyloen' was 2n=48=32m+12sm+4st(2SAT)and 2n=48=34m+10sm+4st(2SAT), respectively. The chromosome composition of relative length was 2n=48=12L+10M2+14M1+12S and 2n=48=12L+8M2+16M1+12S, respectively. The asymmetrical karyotype coefficients of Ph. edulis and Ph. edulis ‘Pachyloen' were 61.41% and 59.55%, and their karyotypes were both of 2B type. The results showed that there were 2 loci of 45S rDNA and 4 loci of 5S rDNA in both Ph. edulis and Ph. edulis ‘Pachyloen'. 【Conclusion】The chromosome number in the two materials is 2n=48, the 45S and 5S rDNA loci of the two materials have no obvious differences, and the two varieties have similar karyotypes, which shows that they have close genetic relatedness.

CLC Number:

HAN Yanli,GUO Qirong, HAN Yonghua, YU Zhaoyuan,MI Yueyin. The karyotype and FISH analysis of 45S and 5S rDNA on the chromosomes of Phyllostachys edulis and Phyllostachys edulis ‘Pachyloen'[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(02): 203-208.

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The karyotype and FISH analysis of 45S and 5S rDNA on the chromosomes of Phyllostachys edulis and Phyllostachys edulis ‘Pachyloen'

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