Purification of total triterpenoids from lingonberry and anti-inflammatory activity in vitro

doi:10.3969/j.issn.1000-2006.201809010

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 62 ›› Issue (04): 132-138.doi: 10.3969/j.issn.1000-2006.201809010

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Purification of total triterpenoids from lingonberry and anti-inflammatory activity in vitro

FAN Ziluan^1,2, ZHAO Zirou^l, ZHAO Xiang¹, CHEN Kaili¹, FU Yujie^3*

(1. College of Forestry,Northeast Forestry University, Harbin 150040, China; 2. Key Laboratory of Forest Food Resources Utilization of Heilongjiang Province, Harbin 150040, China; 3. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China)

Online:2019-07-22 Published:2019-07-22

Abstract

Abstract: 【Objective】 In order to further develop and utilize lingonberry(Vaccinium vitis-idaea)fruits, the total triterpenoids of lingonberry were obtained, and the purification process was optimized through using macroporous adsorption resin and secondary purification of with Sephadex LH-20. The in vitro anti-inflammatory activity of this triterpene compound was analyzed. 【Method】 The experiment was based on the wild buckwheat-hung lingonberry as the raw material. The a static adsorption-analysis experiment, and the dynamic adsorption -analysis experiment were used to select the macroporous adsorption resin, optimization of the best process, and determination of the best sample concentration, pH 6, sample volume, sample flow rate, and ethanol concentration of the eluent. The secondary purification used Sephadex LH-20 hydroxypropyl dextran gel to obtain high-purity lingonberry total triterpene. Using hyaluronidase and the inhibition of serum albumin denaturation as an anti-inflammatory evaluation index to evaluate the anti-inflammatory activity of the total triterpenes of from lingonberry. 【Result】 The purification effect of seven macroporous resins on the total triterpenes of lingonberry was compared. The results showed that the X-5 resin was most suitable for the primary purification. The optimum purification process was the sample concentration of 1.5 mg/mL, elution at pH 6, loading volume 4/3 BV, loading flow rate of 1 BV/h, and 80% ethanol as eluant. The purity of the total triterpenes of lingonberry increased from 5.13% to 29.46%. Further purification using Sephadex LH-20 obtained a purity of(43.25±0.31)%. The total anti-inflammatory activity of the lingonberry was(81.5±1.37)% for the hyaluronidase and(72.59±1.84)% for the denaturation of bovine serum albumin. 【Conclusion】 Lingonberry is a nutrient-rich berry. High-purity triterpenoids were obtained through the secondary purification technology, and it was preliminarily showed that the total triterpenes of lingonberry had significant anti-inflammatory activity. Our results provided a certain theoretical basis for the further development and utilization of lingonberry.

CLC Number:

TS255.4

FAN Ziluan, ZHAO Zirou^l, ZHAO Xiang, CHEN Kaili, FU Yujie. Purification of total triterpenoids from lingonberry and anti-inflammatory activity in vitro[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 62(04): 132-138.

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Purification of total triterpenoids from lingonberry and anti-inflammatory activity in vitro

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