Anatomical observation and three-dimensional construction of leaf blades from six bamboos

doi:10.3969/j.issn.1000-2006.201810044

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (1): 47-53.doi: 10.3969/j.issn.1000-2006.201810044

Anatomical observation and three-dimensional construction of leaf blades from six bamboos

SU Jialu¹^,²^,³(), LIN Shuyan¹^,²^,^*(), SHI Wushuang¹^,²^,³, WANG Xing¹^,²^,³, ZHENG Xiao¹^,²^,³, WAN Yawen¹^,²^,³, DING Yulong¹^,²

1. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
2. Bamboo Research Institute, Nanjing Forestry University, Nanjing 210037, China
3. College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China

Received:2018-10-31 Revised:2018-11-10 Online:2020-02-08 Published:2020-02-02
Contact: LIN Shuyan E-mail:sujialu1023@163.com;lrx@njfu.com.cn

Abstract

Abstract:

【Objective】 To compare the differences in leaf structure and understand the three-dimensional images of the internal anatomical structures of six bamboo species/cultivars. 【Method】 The leaves of six bamboo species/cultivars were collected, including Indosasa hispida ‘Rainbow’, Sasaella glabra f. albostriata, Pleioblastus fortunei, Hibanobambusa tranquillans f. shiroshima, Sasaella kongosanensis ‘Aureostriatus’ and Sinobambusa tootsik var. luteoloalbostriata. For each species/cultivars, the leaf anatomical structures (transverse section: perpendicular to the transverse section of the midrib, longitudinal section: parallel to the longitudinal section of the midrib, and another section: parallel to the leaf epidermis) were obtained by paraffin sectioning and photographed under light microscopy. Then, the three-dimensional inner structures of the leaves were generated in Photoshop.【Result】 The epidermal system of the six bamboo species/cultivars was similarly structured. Most of the bulliform cells were arranged in 2-5 rows; and the short cells were concentrated near the vein area of the upper epidermis. However, there were significant differences in the basic structure of the leaf tissue system. The number of layers and the size of the mesophyll cells among the six bamboo species/cultivars were different, though the arrangement of the mesophyll cells was regular. There were two types of fusoid cells among the six species/cultivars: apoptotic and viable. The fusoid cells were apoptotic in the leaves ofIn. hispida ‘Rainbow’, most fusoid cells were apoptotic in the leaves of Pl. fortunei, H. tranquillans f. shiroshima and S. tootsik var. luteoloalbostriata, while few of the fusoid cells were apoptotic in the leaves of S. kongosanensis ‘Aureostriatus’ and S. glabra f. albostriata. The vascular system consists of the midrib, primary vein, secondary vein, and minor transverse veins. 【Conclusion】 The establishment of the three-dimensional structure of bamboo leaves helps us understand the internal structure better and provides us a reference for further classification and system evolution in bamboo.

Key words: ornamental bamboo, bamboo leaves, anatomical characteristics, three-dimensional structure

CLC Number:

S718

SU Jialu, LIN Shuyan, SHI Wushuang, WANG Xing, ZHENG Xiao, WAN Yawen, DING Yulong. Anatomical observation and three-dimensional construction of leaf blades from six bamboos[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(1): 47-53.

Figures/Tables 4

Fig.1

Table 1

Table 2

Table 3

References 28

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竹种/品种 bamboo species/ cultivar	表皮厚度/μm epidermis thickness		长细胞长度/μm long cell length	短细胞数量 short cell	泡状细胞组 the bulliform cells
竹种/品种 bamboo species/ cultivar	上表皮 upper	下表皮 lower	长细胞长度/μm long cell length	短细胞数量 short cell	长/μm length	宽/μm width
‘七彩红竹’	10.50±0.99 d	8.58±1.17 d	98.16±24.57 a	脉区较多分布,脉间少量分布	44.86±5.42 b	59.35± 5.30 c
菲白竹	12.91±1.75 c	9.30±1.39 d	68.94±16.79 c	脉区集中分布,脉间少量分布	44.53±4.87 b	72.98±10.00 a
靓竹	15.98±2.01 a	12.38±2.14 b	63.92±17.89 c	脉区脉间随机分布	47.75±8.46 b	65.33±10.07 b
‘黄条金刚竹’	13.52±1.47 c	11.48±1.90 bc	94.06±21.71 a	脉区较多分布,脉间少量分布	53.30±4.28 a	75.95±8.20 a
锦竹	16.49±2.30 a	13.63±2.57 a	83.24±22.24 b	脉区较多分布,脉间少量分布	46.21±7.40 b	71.24±11.93 a
花叶唐竹	14.42±1.31 b	11.23±1.94 c	74.58±13.37 bc	脉区较多分布,脉间少量分布	37.41±5.68 c	76.43±8.51 a

竹种/品种 bamboo species/ cultivar	指状臂细胞厚度 arm cell thickness	梅花状细胞厚度 rosette cell thickness	梭型细胞长度 fusoid cell length	叶片厚度 leaf thickness
‘七彩红竹’	20.70±1.68 d	14.06±2.17 c	86.90±6.36 a	85.50±13.75 f
菲白竹	23.71±2.15 c	18.56±1.87 b	61.61±12.80 b	116.48 ±20.83 d
靓竹	27.12±3.43 ab	19.64± 3.08 ab	45.11±7.14 d	148.05±8.06 a
‘黄条金刚竹’	26.58±3.59 b	18.40±2.51 b	50.30±7.30 cd	128.57±8.79 c
锦竹	28.55±5.03 a	20.26±3.51 a	66.64±6.87 b	140.11±6.63 b
花叶唐竹	23.92±4.04 c	13.65±1.74 c	54.32±9.95 c	104.29±6.77 e

竹种/品种 bamboo species/ cultivar	一级脉 first vascular		二级脉横截面积/μm² area of second vascular	横脉横截面积/μm² area of transverse vascular	二级脉间距/μm distance between second vascular
竹种/品种 bamboo species/ cultivar	数量 number	横截面积/μm² area	二级脉横截面积/μm² area of second vascular	横脉横截面积/μm² area of transverse vascular	二级脉间距/μm distance between second vascular
‘七彩红竹’	14~18	7 434.26±931.09 ab	2 235.06±212.00 a	453.72±80.22 a	210.91±18.71 a
菲白竹	8~10	5 557.01±754.38 c	1 872.54±160.40 b	446.83±42.15 a	162.85±34.51 cd
靓竹	8~10	6 621.92±638.44 b	2 028.52±330.86 ab	362.86±84.91 b	134.99±23.99 d
‘黄条金刚竹’	10~12	8 050.18±382.74 a	1 820.15±334.88 b	369.76±71.13 b	156.31±14.43 d
锦竹	12~14	7 545.59±628.31 a	1 913.39±496.72 b	374.55±105.3 b	194.20±15.27 b
花叶唐竹	6~8	3 833.54±410.90 d	1 542.28±198.71 c	221.73±38.61 c	174.59±12.54 c

Anatomical observation and three-dimensional construction of leaf blades from six bamboos

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