Microscopic observation on the process of axenic seed germination of Cymbidium tortisepalum var. longibracteatum

doi:10.3969/j.issn.1000-2006.201812040

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (2): 105-110.doi: 10.3969/j.issn.1000-2006.201812040

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Microscopic observation on the process of axenic seed germination of Cymbidium tortisepalum var. longibracteatum

ZHANG Qiao(), LI Jie^*(), SHAN Mengmeng, LIU Xin, LI Yuanyuan

College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010,China

Received:2018-12-23 Revised:2019-07-24 Online:2020-03-30 Published:2020-04-01
Contact: LI Jie E-mail:1107065389@qq.com;jay0224@sina.com

Abstract

Abstract:

【Objective】It’s difficult to germinate for the seed ofCymbidium tortisepalum var. longibracteatum with only about one millionth germination rate in the wild. 【Method】The axenic sowing technology is the way to seed germinationtests for Cymbidium tortisepalum var. longibracteatum. Meanwhile, fluorescent inverted microscope and scanning electron microscope are the principal tools to observe and study the seed morphological changes in every developmental stages. 【Result】Results indicated that the best germination culture medium is Hyponex 3 g/L+6-BA 0.50 mg/L + NAA 0.50 mg/L and the germination rate is about 30.33%, spending 400 days from sowing to seedling forming. As observation revealed, the embryo expands and breaks through the testa from one side of the seed without separating from the testa, the embryo turns green through photosynthesis and differentiate into the protocorms after breaking through the testa,and the protocorm elongats and grows into rhizome and finally form seedling. 【Conclusion】The appropriate concentration of 6-BA and NAA can effectively promote germination of Cymbidium tortisepalum var. longibracteatum and the seed development stages of Cymbidium tortisepalum var. longibracteatum includes: ungermination, the embryo absorbs water and expands,the testa is teared with the embryo visible, the formation of protocorm, the formation of rhizome, and the formation of seedling. The results can provide reference for the efficient germination and symbiotic germination of Cymbidium tortisepalum var. longibracteatum.

Key words: Cymbidium tortisepalum var. longibracteatum, seed, axenic seed germination, morphological changes

CLC Number:

S682.31

ZHANG Qiao, LI Jie, SHAN Mengmeng, LIU Xin, LI Yuanyuan. Microscopic observation on the process of axenic seed germination of Cymbidium tortisepalum var. longibracteatum[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(2): 105-110.

Figures/Tables 5

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References 21

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培养基序号 No. of culture medium	激素质量浓度/(mg·L^-1) concentrations of hormone			萌发率/% germination rate	生长情况 growth situation
培养基序号 No. of culture medium	NAA		6-BA	萌发率/% germination rate	生长情况 growth situation
C	0.50	0.50		30.33±4.40 a	根状茎健壮,分化好,呈绿色 rhizomes are strong, well differentiated and green
D	0.75	0.75		24.00±2.52 b	根状茎弱小,分化次之,呈浅绿色 rhizomes are small,differentiated secondly, and light green
E	1.00	1.00		14.89±1.26 c	原球茎嫩绿色,无根状茎 protocorms are tender green without rhizomes
B	0.25	0.25		8.89±1.45 d	原球茎嫩绿色,无根状茎 protocorms are tender green without rhizomes
A(CK)	0.00	0.00		0.42±0.05 e	尚未形成原球茎 no protocorms

发育阶段 the development stages	描述 description
1	未萌发,种子种皮完整 seeds do not germinate and the testae are complete
2	种胚吸水膨大 embryos absorb water and expand
3	种皮破裂,露出种胚 testae are teared with the embryos visible
4	形成原球茎 formation of protocorms
5	形成根状茎 formation of rhizomes
6	形成幼苗 formation of seedlings

Microscopic observation on the process of axenic seed germination of Cymbidium tortisepalum var. longibracteatum

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